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Literature summary extracted from

  • Marco-Marin, C.; Ramon-Maiques, S.; Tavarez, S.; Rubio, V.
    Site-directed mutagenesis of Escherichia coli acetylglutamate kinase and aspartokinase III probes the catalytic and substrate-binding mechanisms of these amino acid kinase family enzymes and allows three-dimensional modelling of aspartokinase (2003), J. Mol. Biol., 334, 459-476.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.7.2.8 D162E about 0.1% of wild-type activity Escherichia coli
2.7.2.8 K8R substantial although diminished activity Escherichia coli
2.7.2.8 N158K substantial although diminished activity Escherichia coli
2.7.2.8 R66K substantial although diminished activity Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.2.8 ATP
-
Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.2.8 0.2
-
N-acetyl-L-glutamate wild-type, pH 7.0, 25°C Escherichia coli
2.7.2.8 0.29
-
ATP wild-type, pH 7.0, 25°C Escherichia coli
2.7.2.8 0.37
-
N-acetyl-L-glutamate mutant D162E, pH 7.0, 25°C Escherichia coli
2.7.2.8 0.46
-
ATP mutant D162E, pH 7.0, 25°C Escherichia coli
2.7.2.8 2.3
-
N-acetyl-L-glutamate mutant K8R, pH 7.0, 25°C Escherichia coli
2.7.2.8 3.3
-
ATP mutant K8R, pH 7.0, 25°C Escherichia coli
2.7.2.8 5.2
-
ATP mutant R66K, pH 7.0, 25°C Escherichia coli
2.7.2.8 7.9
-
ATP mutant N158Q, pH 7.0, 25°C Escherichia coli
2.7.2.8 600
-
N-acetyl-L-glutamate mutant N158Q, pH 7.0, 25°C Escherichia coli
2.7.2.8 898
-
N-acetyl-L-glutamate mutant R66K, pH 7.0, 25°C Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.8 Escherichia coli P0A6C8
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.2.8 ATP + N-acetyl-L-glutamate
-
Escherichia coli ADP + N-acetyl-L-glutamate 5-phosphate
-
?

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.2.8 30.4
-
ATP wild-type, pH 7.0, 25°C Escherichia coli
2.7.2.8 41.5
-
ATP mutant N158Q, pH 7.0, 25°C Escherichia coli