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Literature summary extracted from

  • Yun, S.H.; Yun, C.Y.; Kim, S.I.
    Characterization of protocatechuate 4,5-dioxygenase induced from p-hydroxybenzoate-cultured Pseudomonas sp. K82 (2004), J. Microbiol., 42, 152-155.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.13.11.8 rapid loss of activity during purification due to the oxidation of an active-site ferrous iron group Pseudomonas sp. K82

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.8 H2O2
-
 Pseudomonas sp. K82

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.8 Fe2+
-
 Pseudomonas sp. K82
1.13.11.8 Mn2+
-
 Pseudomonas sp. K82

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.8 17000
-
 1 * 17000, + 1 * 34000, SDS-PAGE Pseudomonas sp. K82
1.13.11.8 34000
-
 1 * 17000, + 1 * 34000, SDS-PAGE Pseudomonas sp. K82
1.13.11.8 54000
-
 gel filtration Pseudomonas sp. K82

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.8 protocatechuate + O2 Pseudomonas sp. K82
-
 4-carboxy-2-hydroxymuconate semialdehyde
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.8 Pseudomonas sp. K82
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.8 ammonium sulfate, Q-Sepharose Pseudomonas sp. K82

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.8 0.14
-
-
 Pseudomonas sp. K82

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.8 protocatechuate + O2
-
 Pseudomonas sp. K82 4-carboxy-2-hydroxymuconate semialdehyde
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.8 dimer 1 * 17000, + 1 * 34000, SDS-PAGE Pseudomonas sp. K82

Synonyms

EC Number Synonyms Comment Organism
1.13.11.8 PCD 4,5
-
 Pseudomonas sp. K82

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.8 15
-
-
 Pseudomonas sp. K82

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.13.11.8 5 35 approx. 85% of maximal activity at 5°C, approx. 20% of maximal activity at 35°C Pseudomonas sp. K82

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.8 8.8 9
-
 Pseudomonas sp. K82