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Literature summary extracted from

  • Liu, D.; Nagan, N.; Just, W.W.; Rodemer, C.; Thai, T.P.; Zoeller, R.A.
    Role of dihydroxyacetonephosphate acyltransferase in the biosynthesis of plasmalogens and nonether glycerolipids (2005), J. Lipid Res., 46, 727-735.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.42 expression in CHO cell line NRe1-4 with severe enzyme activity defects restores the plasmalogen biosynthesis activity to 10% of wild-type activity, overexpression of the human enzyme leads to 6fold increased enzyme activity and 55% restored plasmalogen synthesizing activity, nonether glycerolipid biosynthesis is unaffected or dcreased in both cases Homo sapiens
2.3.1.42 expression of the human enzyme encoding cDNA in a CHO cell line NRe1-4 with severe enzyme activity defects restores the plasmalogen biosynthesis activity to 10% of wild-type activity, overexpression of the human enzyme leads to 6fold increased enzyme activity and 55% restored plasmalogen synthesizing activity, nonether glycerolipid biosynthesis is unaffected or decreased in both cases Cricetulus griseus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.42 peroxisome
-
 Cricetulus griseus 5777
-
2.3.1.42 peroxisome
-
 Homo sapiens 5777
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.42 acyl-CoA + glycerone phosphate Cricetulus griseus enzyme is essential for biosynthesis of plasmalogens and nonetherlipids, the peroxisomal isozymes does not contribute to nonetherlipid biosynthesis CoA + acylglycerone phosphate
-
 ?
2.3.1.42 acyl-CoA + glycerone phosphate Homo sapiens enzyme is essential for biosynthesis of plasmalogens and nonetherlipids, the peroxisomal isozymes does not contribute to nonetherlipid biosynthesis CoA + acylglycerone phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.42 Cricetulus griseus
-
-
-
2.3.1.42 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.42 cell culture CHO-K1 cell line Cricetulus griseus
-
2.3.1.42 CHO-K1 cell
-
 Cricetulus griseus
-
2.3.1.42 ovary
-
 Cricetulus griseus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.42 0.00004 0.00008 CHO-NRe1-4 cell expressing the human enzyme Cricetulus griseus
2.3.1.42 0.00004 0.00008 CHO-NRe1-4 cell expressing the human enzyme Homo sapiens
2.3.1.42 0.00042
-
 CHO-K1 wild-type cell Cricetulus griseus
2.3.1.42 0.00245
-
 CHO-NRe1-4 cell overexpressing the human enzyme Cricetulus griseus
2.3.1.42 0.00245
-
 CHO-NRe1-4 cell overexpressing the human enzyme Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.42 acyl-CoA + glycerone phosphate
-
 Cricetulus griseus CoA + acylglycerone phosphate
-
 ?
2.3.1.42 acyl-CoA + glycerone phosphate
-
 Homo sapiens CoA + acylglycerone phosphate
-
 ?
2.3.1.42 acyl-CoA + glycerone phosphate enzyme is essential for biosynthesis of plasmalogens and nonetherlipids, the peroxisomal isozymes does not contribute to nonetherlipid biosynthesis Cricetulus griseus CoA + acylglycerone phosphate
-
 ?
2.3.1.42 acyl-CoA + glycerone phosphate enzyme is essential for biosynthesis of plasmalogens and nonetherlipids, the peroxisomal isozymes does not contribute to nonetherlipid biosynthesis Homo sapiens CoA + acylglycerone phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.42 DHAPAT
-
 Cricetulus griseus
2.3.1.42 DHAPAT
-
 Homo sapiens
2.3.1.42 dihydroxyacetonephosphate acyltransferase
-
 Cricetulus griseus
2.3.1.42 dihydroxyacetonephosphate acyltransferase
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.42 37
-
 assay at Cricetulus griseus
2.3.1.42 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.42 5.5
-
-
 Cricetulus griseus
2.3.1.42 5.5
-
-
 Homo sapiens