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Literature summary extracted from

  • Dhiman, R.K.; Schulbach, M.C.; Mahapatra, S.; Baulard, A.R.; Vissa, V.; Brennan, P.J.; Crick, D.C.
    Identification of a novel class of omega,E,E-farnesyl diphosphate synthase from Mycobacterium tuberculosis (2004), J. Lipid Res., 45, 1140-1147.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.5.1.10 dithiothreitol 2.5 mM, increases activity 2fold Mycobacterium tuberculosis
2.5.1.10 Triton X-100 0.2%, 1.5fold increase in activity Mycobacterium tuberculosis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.10 expression in Mycobacterium smegmatis using a new mycobacterial expression vector that encodes an in-frame N-terminal affinity tag fusion with the protein of interest Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.10 Triton X-100 above 0.2% Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.10 0.0098
-
 geranyl diphosphate pH 7.9, 37°C Mycobacterium tuberculosis
2.5.1.10 0.043
-
 isopentenyl diphosphate pH 7.9, 37°C Mycobacterium tuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.10 Fe2+ absolutely dependent on presence of divalent cation. Supports activity to a lower level than Mg2+ Mycobacterium tuberculosis
2.5.1.10 Mg2+ absolutely dependent on presence of divalent cation. Optimal activity at 4-10 mM Mycobacterium tuberculosis
2.5.1.10 Mn2+ absolutely dependent on presence of divalent cation. Supports activity to a lower level than Mg2+ Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.10 Mycobacterium tuberculosis
-
-
-
2.5.1.10 Mycobacterium tuberculosis H37Rv
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.10 fusion protein Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.10 geranyl diphosphate + isopentenyl diphosphate no activity with dimethylallyl diphosphate Mycobacterium tuberculosis diphosphate + trans,trans-farnesyl diphosphate
-
 ?
2.5.1.10 geranyl diphosphate + isopentenyl diphosphate no activity with dimethylallyl diphosphate Mycobacterium tuberculosis H37Rv diphosphate + trans,trans-farnesyl diphosphate
-
 ?
2.5.1.10 additional information no activity with dimethylallyl diphosphate Mycobacterium tuberculosis ?
-
 ?
2.5.1.10 additional information no activity with dimethylallyl diphosphate Mycobacterium tuberculosis H37Rv ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.10 omega,E,E-farnesyl diphosphate synthase
-
 Mycobacterium tuberculosis
2.5.1.10 omega,E,E-FPP
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.10 0.2
-
 geranyl diphosphate pH 7.9, 37°C Mycobacterium tuberculosis
2.5.1.10 0.49
-
 isopentenyl diphosphate pH 7.9, 37°C Mycobacterium tuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.10 8
-
-
 Mycobacterium tuberculosis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.10 7 10 active over the range pH 7-10 Mycobacterium tuberculosis