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Literature summary extracted from

  • Ye, H.Q.; Mallonee, D.H.; Wells, J.E.; Bjorkhem, I.; Hylemon, P.B.
    The bile acid-inducible baiF gene from Eubacterium sp. strain VPI 12708 encodes a bile acid-coenzyme A hydrolase (1999), J. Lipid Res., 40, 17-23.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.2.27 expression in Escherichia coli Eubacterium sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.2.27 0.175
-
 cholyl-CoA pH 7.0, 37°C Eubacterium sp.

Organism

EC Number Organism UniProt Comment Textmining
3.1.2.27 Eubacterium sp.
-
-
-
3.1.2.27 Eubacterium sp. VPI 12708
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.2.27 recombinant Eubacterium sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.2.27 61
-
-
 Eubacterium sp.

Storage Stability

EC Number Storage Stability Organism
3.1.2.27 -20°C, enzyme is stable for several months in 50% glycerol Eubacterium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.27 3-dehydrocholoyl-CoA + H2O
-
 Eubacterium sp. 3-dehydrocholate + CoA
-
 ?
3.1.2.27 3-dehydrocholoyl-CoA + H2O
-
 Eubacterium sp. VPI 12708 3-dehydrocholate + CoA
-
 ?
3.1.2.27 chenodeoxycholoyl-CoA + H2O
-
 Eubacterium sp. chenodeoxycholate + CoA
-
 ?
3.1.2.27 chenodeoxycholoyl-CoA + H2O
-
 Eubacterium sp. VPI 12708 chenodeoxycholate + CoA
-
 ?
3.1.2.27 choloyl-CoA + H2O
-
 Eubacterium sp. cholate + CoA
-
 ?
3.1.2.27 choloyl-CoA + H2O
-
 Eubacterium sp. VPI 12708 cholate + CoA
-
 ?
3.1.2.27 additional information no activity with acetyl-CoA, isovaleryl-CoA, palmitoyl-CoA or phenylacetyl-CoA are used as substrates Eubacterium sp. ?
-
 ?
3.1.2.27 additional information no activity with acetyl-CoA, isovaleryl-CoA, palmitoyl-CoA or phenylacetyl-CoA are used as substrates Eubacterium sp. VPI 12708 ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.2.27 6 7
-
 Eubacterium sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.2.27 4 9 no activity detected below pH 4.0 or above pH 9.0 Eubacterium sp.