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Literature summary extracted from

  • Tanaka, N.; Wever, R.
    Inhibition of vanadium chloroperoxidase from the fungus Curvularia inaequalis by hydroxylamine, hydrazine and azide and inactivation by phosphate (2004), J. Inorg. Biochem., 98, 625-631.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.11.1.B2 azide uncompetitive; uncompetitive inhibition, inhibition constant: 50 nM Curvularia inaequalis
1.11.1.B2 hydrazine uncompetitive; uncompetitive inhibition, inhibition constant: 0.3 mM Curvularia inaequalis
1.11.1.B2 hydroxylamine competitive and uncompetitive inhibition; inhibits rVCPO both competitively and uncompetitively. The competitive inhibition constant: 0.04 mM, uncompetitive inhibition 0.08 mM Curvularia inaequalis
1.11.1.B2 phosphate substitution of vanadate in the active site by phosphate leads to inactivation of the enzyme. Presence of H2O2 prevents inactivation by phosphate; substitution of vanadate in the active site by phosphate leads to inactivation. Presence of H2O2 prevents the inactivation Curvularia inaequalis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.11.1.B2 Vanadium substitution of vanadate in the active site by phosphate leads to inactivation of the enzyme. Pervanadate is bound much more strongly to the enzyme than vanadate Curvularia inaequalis

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.B2 Curvularia inaequalis P49053
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.B2 Br- + H2O2 + 1,1-dimethyl-4-chloro-3,5-cyclohexanedione i.e. monochlorodimedone Curvularia inaequalis ?
-
 ?
1.11.1.B2 Cl- + H2O2 + 1,1-dimethyl-4-chloro-3,5-cyclohexanedione i.e. monochlorodimedone Curvularia inaequalis ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.11.1.B2 vanadium chloroperoxidase
-
 Curvularia inaequalis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.11.1.B2 0.04
-
 hydroxylamine competitive inhibition constant Curvularia inaequalis
1.11.1.B2 0.08
-
 hydroxylamine uncompetitive inhibition constant Curvularia inaequalis
1.11.1.B2 0.35
-
 hydrazine
-
 Curvularia inaequalis