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Literature summary extracted from

  • Lunn, J.E.; Gillespie, V.J.; Furbank, R.T.
    Expression of a cyanobacterial sucrose-phosphate synthase from Synechocystis sp. PCC 6803 in transgenic plants (2003), J. Exp. Bot., 54, 223-237.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.14 additional information recombinant enzyme expressed in Escherichia coli is not activated by glucose 6-phosphate Synechocystis sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.14 expression in Escherichia coli. The Synechocystis sps gene is introduced into tobacco, rice and tomato under the control of constitutive promoters. The Synechocystis SPS protein is expressed at high level. However SPS activities and carbon partitioning in leaves from transgenic and wild-type plants are not significantly different. The purified enzymes have full catalytic activity. It is proposed that some other protein in plant cells binds to the Synechocystis SPS resulting in inhibition of the enzyme Synechocystis sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.14 additional information the Synechocystis sps gene is introduced into tobacco, rice and tomato under the control of constitutive promoters. The Synechocystis SPS protein is expressed at high level. However SPS activities and carbon partitioning in leaves from transgenic and wild-type plants are not significantly different. The purified enzymes have full catalytic activity. It is proposed that some other protein in plant cells binds to the Synechocystis SPS resulting in inhibition of the enzyme Synechocystis sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.14 0.2
-
 D-fructose 6-phosphate pH 8.0, cosubstrate: UDP-glucose, enzyme expressed in Escherichia coli Synechocystis sp.
2.4.1.14 0.3
-
 D-fructose 6-phosphate pH 8.0, cosubstrate: UDP-glucose, enzyme expressed in Nicotiana tabacum Synechocystis sp.
2.4.1.14 1.5
-
 GDP-glucose pH 8.0, enzyme expressed in Escherichia coli Synechocystis sp.
2.4.1.14 1.8
-
 UDP-glucose pH 8.0, enzyme expressed in Nicotiana tabacum Synechocystis sp.
2.4.1.14 2
-
 ADP-glucose pH 8.0, enzyme expressed in Nicotiana tabacum Synechocystis sp.
2.4.1.14 2.2
-
 UDP-glucose pH 8.0, enzyme expressed in Escherichia coli Synechocystis sp.
2.4.1.14 2.4
-
 GDP-glucose pH 8.0, enzyme expressed in Nicotiana tabacum Synechocystis sp.
2.4.1.14 2.7
-
 ADP-glucose pH 8.0, enzyme expressed in Escherichia coli Synechocystis sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.14 82000
-
 x * 82000, SDS-PAGE Synechocystis sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.14 UDP-alpha-D-glucose + D-fructose 6-phosphate Synechocystis sp.
-
 UDP + sucrose 6-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.14 Synechocystis sp.
-
 PCC 6803
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.14 transgenic SPS from tobacco and rice Synechocystis sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.14 ADP-glucose + D-fructose 6-phosphate
-
 Synechocystis sp. ADP + sucrose 6-phosphate
-
 ?
2.4.1.14 GDP-glucose + D-fructose 6-phosphate
-
 Synechocystis sp. GDP + sucrose 6-phosphate
-
 ?
2.4.1.14 UDP-alpha-D-glucose + D-fructose 6-phosphate
-
 Synechocystis sp. UDP + sucrose 6-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.14 ? x * 82000, SDS-PAGE Synechocystis sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.14 8.5
-
 recombinant enzyme expressed in Escherichia coli Synechocystis sp.