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Literature summary extracted from

  • Minami, Y.; Kohama, T.; Sekimoto, Y.J.; Akasaka, K.; Matsubara, H.
    Isolation and characterization of glutathione reductase from Physarum polycephalum and stage-specific expression of the enzyme in life-cycle stages with different oxidation-reduction levels (2003), J. Eukaryot. Microbiol., 50, 317-323.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.1.7 construction of a genetic library, DNA and amino acid sequence determination and analysis of the enzyme encoding gene, expression in Escherichia coli Physarum polycephalum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.1.7 0.0286
-
 NADPH
-
 Physarum polycephalum
1.8.1.7 0.04
-
 glutathione disulfide
-
 Physarum polycephalum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.1.7 49300
-
 2 * 50000, SDS-PAGE, 2 * 49300, MALDI-TOF mass spectrometry Physarum polycephalum
1.8.1.7 50000
-
 2 * 50000, SDS-PAGE, 2 * 49300, MALDI-TOF mass spectrometry Physarum polycephalum
1.8.1.7 99200
-
 velocity sedimentation analysis Physarum polycephalum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.1.7 glutathione disulfide + NADPH Physarum polycephalum
-
 glutathione + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.7 Physarum polycephalum Q9NL96 strain ATCC 24466 and 24467
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.1.7 native enzyme from microplasmodia, 968fold in a multistep procedure Physarum polycephalum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.8.1.7 microplasmodium
-
 Physarum polycephalum
-
1.8.1.7 additional information stage-specific expression of the enzyme in life-cycle stages with different oxidation-reduction levels, glutathione levels, overview Physarum polycephalum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.1.7 484
-
 purified native enzyme Physarum polycephalum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.7 glutathione disulfide + NADPH
-
 Physarum polycephalum glutathione + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.1.7 dimer 2 * 50000, SDS-PAGE, 2 * 49300, MALDI-TOF mass spectrometry Physarum polycephalum

Synonyms

EC Number Synonyms Comment Organism
1.8.1.7 glutathione reductase
-
 Physarum polycephalum
1.8.1.7 GR
-
 Physarum polycephalum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.8.1.7 37
-
 assay at Physarum polycephalum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.1.7 6.5
-
-
 Physarum polycephalum

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.7 NADPH
-
 Physarum polycephalum