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Literature summary extracted from

  • Benboubetra, M.; Baghiani, A.; Atmani, D.; Harrison, R.
    Physicochemical and kinetic properties of purified sheep's milk xanthine oxidoreductase (2004), J. Dairy Sci., 87, 1580-1584.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.17.1.4 0.00215
-
 xanthine pH 7.2, 25°C Bos taurus
1.17.1.4 0.00219
-
 NAD+ pH 7.2, 25°C Ovis aries
1.17.1.4 0.00252
-
 NAD+ pH 7.2, 25°C Homo sapiens
1.17.1.4 0.00274
-
 NAD+ pH 7.2, 25°C Bos taurus
1.17.1.4 0.00412
-
 NAD+ pH 7.2, 25°C Capra hircus
1.17.1.4 0.00633
-
 xanthine pH 7.2, 25°C Capra hircus
1.17.1.4 0.00714
-
 xanthine pH 7.2, 25°C Ovis aries
1.17.1.4 0.00774
-
 xanthine pH 7.2, 25°C Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.17.1.4 Molybdenum 0.18 atoms per subunit Ovis aries

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.17.1.4 150000
-
 x * 150000, SDS-PAGE Ovis aries

Organism

EC Number Organism UniProt Comment Textmining
1.17.1.4 Bos taurus
-
-
-
1.17.1.4 Capra hircus
-
-
-
1.17.1.4 Homo sapiens
-
-
-
1.17.1.4 Ovis aries
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.17.1.4
-
 Ovis aries

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.17.1.4 milk
-
 Homo sapiens
-
1.17.1.4 milk
-
 Bos taurus
-
1.17.1.4 milk
-
 Ovis aries
-
1.17.1.4 milk
-
 Capra hircus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.17.1.4 0.06
-
 substrate xanthine, pH 7.2, 25°C Homo sapiens
1.17.1.4 0.21
-
 substrate NAD+, pH 7.2, 25°C Ovis aries
1.17.1.4 0.25
-
 substrate NAD+, pH 7.2, 25°C Bos taurus
1.17.1.4 0.27
-
 substrate NAD+, pH 7.2, 25°C Capra hircus
1.17.1.4 0.27
-
 substrate xanthine, pH 7.2, 25°C Capra hircus
1.17.1.4 0.29
-
 substrate NAD+, pH 7.2, 25°C Homo sapiens
1.17.1.4 0.69
-
 substrate xanthine, pH 7.2, 25°C Ovis aries
1.17.1.4 1.83
-
 substrate xanthine, pH 7.2, 25°C Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.1.4 xanthine + NAD+ + H2O
-
 Homo sapiens urate + NADH
-
 ?
1.17.1.4 xanthine + NAD+ + H2O
-
 Bos taurus urate + NADH
-
 ?
1.17.1.4 xanthine + NAD+ + H2O
-
 Ovis aries urate + NADH
-
 ?
1.17.1.4 xanthine + NAD+ + H2O
-
 Capra hircus urate + NADH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.17.1.4 ? x * 150000, SDS-PAGE Ovis aries