Literature summary extracted from

Chai, S.C.; Jerkins, A.A.; Banik, J.J.; Shalev, I.; Pinkham, J.L.; Uden, P.C.; Maroney, M.J.
Heterologous expression, purification, and characterization of recombinant rat cysteine dioxygenase (2005), J. Biol. Chem., 280, 9865-9869.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
1.13.11.20	expressed as a His-tag fusion protein in Escherichia coli BL21(DE3)pLysS	Rattus norvegicus

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
1.13.11.20	homocysteine	50% inhibition at 6.5 mM	Rattus norvegicus

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.13.11.20	2.5	-	L-cysteine	pH 7.5, 37°C	Rattus norvegicus

Metals/Ions

EC Number	Metals/Ions	Comment	Organism	Structure
1.13.11.20	Iron	loosely bound to protein, only 10% of purified protein contains iron	Rattus norvegicus

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
1.13.11.20	24950	-	mass spectrometry, thrombin cleaved enzyme	Rattus norvegicus
1.13.11.20	26800	-	His-tag fusion protein	Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
1.13.11.20	L-cysteine + O2	Rattus norvegicus	key enzyme of cysteine catabolism, supplies substrate for taurine biosynthesis	3-sulfino-L-alanine	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.13.11.20	Rattus norvegicus	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
1.13.11.20	recombinant protein using His-tag	Rattus norvegicus

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.13.11.20	L-cysteine + O2	-	Rattus norvegicus	3-sulfino-L-alanine	-	?
1.13.11.20	L-cysteine + O2	key enzyme of cysteine catabolism, supplies substrate for taurine biosynthesis	Rattus norvegicus	3-sulfino-L-alanine	-	?

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
1.13.11.20	7.5	-	-	Rattus norvegicus

pH Range

EC Number	pH Minimum	pH Maximum	Comment	Organism
1.13.11.20	6.5	9.5	-	Rattus norvegicus

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Release 2023.1 (January 2023)