EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.1.137 | overexpression of the His-tagged enzyme in Escherichia coli as soluble protein, expression of enzyme for selenomethionine-labeling in Escherichia coli strain B834(DE3) | Mus musculus |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.3.1.137 | purified recombinant His-tagged wild-type and mutants M335V and C323M enzymes free or in complex with octanoylcarnitine, sitting drop vapour diffusion method, 4°C, reservoir solution contains 0.1 M HEPES, pH 7.4, 62% v/v 2-methyl-2,4-pentanediol, larger crystals by micro- and macroseeding, cross-seeding of recombinant purified selenomethionine-labeled enzyme, X-ray diffraction structure determination and analysis at 2.0-2.2 A resolution | Mus musculus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.3.1.137 | C323M | site-directed mutagenesis, crystal structure comparison with the wild-type enzyme, altered acyl-CoA substrate specificity compared to the wild-type enzyme, highly increased specificity for octanoyl-CoA | Mus musculus |
2.3.1.137 | G553M | site-directed mutagenesis, highly reduced activity with altered acyl-CoA substrate specificity compared to the wild-type enzyme | Mus musculus |
2.3.1.137 | M335A | site-directed mutagenesis, reduced activity with altered acyl-CoA substrate specificity compared to the wild-type enzyme | Mus musculus |
2.3.1.137 | M335V | site-directed mutagenesis, crystal structure comparison with the wild-type enzyme, highly reduced activity with altered acyl-CoA substrate specificity compared to the wild-type enzyme | Mus musculus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.137 | octanoyl-CoA + L-carnitine | Mus musculus | - |
CoA + L-octanoylcarnitine | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.137 | Mus musculus | Q9DC50 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.3.1.137 | soluble recombinant His-tagged wild-type and selenomethionine-labeled enzyme from Escherichia coli by nickel affinity chromatography | Mus musculus |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
2.3.1.137 | octanoyl-CoA + L-carnitine = CoA + L-octanoylcarnitine | acyl-binding hydrophobic pocket structure involving residues G553, C323, and Met335, the acyl-CoA substrate chain length specificity is determined by G553, Met335 is important for catalysis | Mus musculus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.3.1.137 | additional information | - |
substrate specificity of wild-type and mutant enzymes, overview | Mus musculus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.137 | acetyl-CoA + L-carnitine | low activity | Mus musculus | CoA + L-acetylcarnitine | - |
? | |
2.3.1.137 | octanoyl-CoA + L-carnitine | - |
Mus musculus | CoA + L-octanoylcarnitine | - |
r | |
2.3.1.137 | octanoyl-CoA + L-carnitine | preferred substrate | Mus musculus | CoA + L-octanoylcarnitine | - |
r | |
2.3.1.137 | tetradecanoyl-CoA + L-carnitine | low activity | Mus musculus | CoA + L-tetradecanoylcarnitine | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.1.137 | carnitine octanoyltransferase | - |
Mus musculus |
2.3.1.137 | CrOT | - |
Mus musculus |
2.3.1.137 | More | the enzyme belongs to the carnitine acyltransferase family | Mus musculus |