BRENDA - Enzyme Database

In vitro phosphorylation by cAMP-dependent protein kinase up-regulates recombinant Saccharomyces cerevisiae mannosylphosphodolichol synthase

Banerjee, D.K.; Carrasquillo, E.A.; Hughey, P.; Schutzbach, J.S.; Martinez, J.A.; Baksi, K.; J. Biol. Chem. 280, 4174-4181 (2005)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.4.1.83
functionally active mutant enzyme S141A is expressed in Escherichia coli
Saccharomyces cerevisiae
Engineering
EC Number
Amino acid exchange
Commentary
Organism
2.4.1.83
S141A
functionally active mutant enzyme with more than 50% reduction in catalytic activity compared to wild-type enzyme
Saccharomyces cerevisiae
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.1.83
additional information
-
additional information
the KM-values for dolichyl phosphate do not differ between the invitro phosphorylated and the control enzyme
Saccharomyces cerevisiae
2.4.1.83
0.0011
-
GDP-mannose
phosphorylated enzyme, 37C, pH 7.0
Saccharomyces cerevisiae
2.4.1.83
0.0012
-
GDP-mannose
non-phosphorylated enzyme, 37C, pH 7.0
Saccharomyces cerevisiae
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.4.1.83
Saccharomyces cerevisiae
P14020
-
-
Posttranslational Modification
EC Number
Posttranslational Modification
Commentary
Organism
2.4.1.83
phosphoprotein
in vitro phosphorylation by cAMP-dependent protein kinase up-regulates recombinant Saccharomyces cerevisiae mannosylphosphodolichol synthase
Saccharomyces cerevisiae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.83
GDP-mannose + dolichyl phosphate
-
659501
Saccharomyces cerevisiae
GDP + dolichyl D-mannosyl phosphate
-
-
-
?
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.4.1.83
12.1
-
GDP-mannose
non-phosphorylated enzyme, 37C, pH 7.0
Saccharomyces cerevisiae
2.4.1.83
70.9
-
GDP-mannose
phosphorylated enzyme, 37C, pH 7.0
Saccharomyces cerevisiae
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.4.1.83
functionally active mutant enzyme S141A is expressed in Escherichia coli
Saccharomyces cerevisiae
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
2.4.1.83
S141A
functionally active mutant enzyme with more than 50% reduction in catalytic activity compared to wild-type enzyme
Saccharomyces cerevisiae
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.1.83
additional information
-
additional information
the KM-values for dolichyl phosphate do not differ between the invitro phosphorylated and the control enzyme
Saccharomyces cerevisiae
2.4.1.83
0.0011
-
GDP-mannose
phosphorylated enzyme, 37C, pH 7.0
Saccharomyces cerevisiae
2.4.1.83
0.0012
-
GDP-mannose
non-phosphorylated enzyme, 37C, pH 7.0
Saccharomyces cerevisiae
Posttranslational Modification (protein specific)
EC Number
Posttranslational Modification
Commentary
Organism
2.4.1.83
phosphoprotein
in vitro phosphorylation by cAMP-dependent protein kinase up-regulates recombinant Saccharomyces cerevisiae mannosylphosphodolichol synthase
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.83
GDP-mannose + dolichyl phosphate
-
659501
Saccharomyces cerevisiae
GDP + dolichyl D-mannosyl phosphate
-
-
-
?
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.4.1.83
12.1
-
GDP-mannose
non-phosphorylated enzyme, 37C, pH 7.0
Saccharomyces cerevisiae
2.4.1.83
70.9
-
GDP-mannose
phosphorylated enzyme, 37C, pH 7.0
Saccharomyces cerevisiae