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Literature summary extracted from

  • Banerjee, D.K.; Carrasquillo, E.A.; Hughey, P.; Schutzbach, J.S.; Martinez, J.A.; Baksi, K.
    In vitro phosphorylation by cAMP-dependent protein kinase up-regulates recombinant Saccharomyces cerevisiae mannosylphosphodolichol synthase (2005), J. Biol. Chem., 280, 4174-4181.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.83 functionally active mutant enzyme S141A is expressed in Escherichia coli Saccharomyces cerevisiae

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.83 S141A functionally active mutant enzyme with more than 50% reduction in catalytic activity compared to wild-type enzyme Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.83 additional information
-
 additional information the KM-values for dolichyl phosphate do not differ between the invitro phosphorylated and the control enzyme Saccharomyces cerevisiae
2.4.1.83 0.0011
-
 GDP-mannose phosphorylated enzyme, 37°C, pH 7.0 Saccharomyces cerevisiae
2.4.1.83 0.0012
-
 GDP-mannose non-phosphorylated enzyme, 37°C, pH 7.0 Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.83 Saccharomyces cerevisiae P14020
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.4.1.83 phosphoprotein in vitro phosphorylation by cAMP-dependent protein kinase up-regulates recombinant Saccharomyces cerevisiae mannosylphosphodolichol synthase Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.83 GDP-mannose + dolichyl phosphate
-
 Saccharomyces cerevisiae GDP + dolichyl D-mannosyl phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.83 Dol-P Man synthase
-
 Saccharomyces cerevisiae
2.4.1.83 DPMS
-
 Saccharomyces cerevisiae
2.4.1.83 mannosylphosphodolichol synthase
-
 Saccharomyces cerevisiae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.83 12.1
-
 GDP-mannose non-phosphorylated enzyme, 37°C, pH 7.0 Saccharomyces cerevisiae
2.4.1.83 70.9
-
 GDP-mannose phosphorylated enzyme, 37°C, pH 7.0 Saccharomyces cerevisiae