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Literature summary extracted from

  • Champagne, K.S.; Sissler, M.; Larrabee, Y.; Doublie, S.; Francklyn, C.S.
    Activation of the hetero-octameric ATP phosphoribosyl transferase through subunit interface rearrangement by a tRNA synthetase paralog (2005), J. Biol. Chem., 280, 34096-34104.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.2.17 ATP binding activates the enzyme complex to the R-state Lactococcus lactis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.17 overexpression of subunits HisGs and HisZ comprising complex in Escherichia coli strain BL21(DE3) as wild-type, or in strain B834 as selenomethionine-labeled complex Lactococcus lactis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.2.17 purified recombinant wild-type and selenomethionine-labeled enzyme complex, the latter additionally by microseeding, hanging drop vapour diffusion method, 0.002 ml well solution containing 15-25% v/v PEG 400, 0.1 M Tris-HCl, pH 7.5, 0.2 M MgCl2, mixed with equal volume of protein solution containing 10-16 mg/ml protein, 10 mM ATP, or 10 mM N-1-methyl-ATP and 5 mM 5-phospho-alpha-D-ribose 1-diphosphate, crystal growth is dependent on ATP or N-1-methyl-ATP, derivatization with 2.5 mM sodium tungstate dihydrate, cryoprotection with 17-18% glycerol, X-ray diffraction structure determination and analysis at 2.9-3.2 A resolution Lactococcus lactis

Protein Variants

EC Number Protein Variants Comment Organism
2.4.2.17 E130A site-directed mutagenesis, about 60% reduced activity compared to the wild-type enzyme, no inhibition by histidine Lactococcus lactis
2.4.2.17 Y268F/Y269F site-directed mutagenesis, about 30% reduced activity compared to the wild-type enzyme, no inhibition by histidine Lactococcus lactis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.17 ADP
-
Lactococcus lactis
2.4.2.17 AMP inhibits the enzyme complex together with histidine to the T-state Lactococcus lactis
2.4.2.17 histidine feedback inhibition, inhibits the enzyme complex together with ATP to the T-state, no inhibition of mutants E130A and Y268F/Y269F Lactococcus lactis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.2.17 Mg2+ dependent on Lactococcus lactis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.17 ATP + 5-phospho-alpha-D-ribose 1-diphosphate Lactococcus lactis first step in histidine biosynthesis 1-(5-phospho-D-ribosyl)-ATP + diphosphate
-
r

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.17 Lactococcus lactis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.17 recombinant wild-type and selenomethionine-labeled enzyme complex comprising subunits HisGs and HisZ from Escherichia coli Lactococcus lactis

Reaction

EC Number Reaction Comment Organism Reaction ID
2.4.2.17 1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate = ATP + 5-phospho-alpha-D-ribose 1-diphosphate substrate binding sites, active site structure, switch between active and inactive conformation Lactococcus lactis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.17 ATP + 5-phospho-alpha-D-ribose 1-diphosphate
-
Lactococcus lactis 1-(5-phospho-D-ribosyl)-ATP + diphosphate
-
r
2.4.2.17 ATP + 5-phospho-alpha-D-ribose 1-diphosphate first step in histidine biosynthesis Lactococcus lactis 1-(5-phospho-D-ribosyl)-ATP + diphosphate
-
r
2.4.2.17 additional information the enzyme comprises 4 catalytic subunits HisGs and 4 regulatory subunits HisZ with histidine as a ligand, 8 histidine binding sites at the subunit interfaces Lactococcus lactis ?
-
?

Subunits

EC Number Subunits Comment Organism
2.4.2.17 More overall structure and monomer architecture, several motif 2 loops in both subunit types, switch structure between active and inactive conformation Lactococcus lactis
2.4.2.17 octamer 4 * catalytic subunit HisGs + 4 * regulatory subunit HisZ, (alpha,beta)4 Lactococcus lactis

Synonyms

EC Number Synonyms Comment Organism
2.4.2.17 ATP phosphoribosyl transferase
-
Lactococcus lactis