Literature summary extracted from
Champagne, K.S.; Sissler, M.; Larrabee, Y.; Doublie, S.; Francklyn, C.S.
Activation of the hetero-octameric ATP phosphoribosyl transferase through subunit interface rearrangement by a tRNA synthetase paralog (2005), J. Biol. Chem., 280, 34096-34104.
Activating Compound
EC Number |
Activating Compound |
Comment |
Organism |
Structure |
---|
2.4.2.17 |
ATP |
binding activates the enzyme complex to the R-state |
Lactococcus lactis |
|
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
2.4.2.17 |
overexpression of subunits HisGs and HisZ comprising complex in Escherichia coli strain BL21(DE3) as wild-type, or in strain B834 as selenomethionine-labeled complex |
Lactococcus lactis |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
2.4.2.17 |
purified recombinant wild-type and selenomethionine-labeled enzyme complex, the latter additionally by microseeding, hanging drop vapour diffusion method, 0.002 ml well solution containing 15-25% v/v PEG 400, 0.1 M Tris-HCl, pH 7.5, 0.2 M MgCl2, mixed with equal volume of protein solution containing 10-16 mg/ml protein, 10 mM ATP, or 10 mM N-1-methyl-ATP and 5 mM 5-phospho-alpha-D-ribose 1-diphosphate, crystal growth is dependent on ATP or N-1-methyl-ATP, derivatization with 2.5 mM sodium tungstate dihydrate, cryoprotection with 17-18% glycerol, X-ray diffraction structure determination and analysis at 2.9-3.2 A resolution |
Lactococcus lactis |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
2.4.2.17 |
E130A |
site-directed mutagenesis, about 60% reduced activity compared to the wild-type enzyme, no inhibition by histidine |
Lactococcus lactis |
2.4.2.17 |
Y268F/Y269F |
site-directed mutagenesis, about 30% reduced activity compared to the wild-type enzyme, no inhibition by histidine |
Lactococcus lactis |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
2.4.2.17 |
ADP |
- |
Lactococcus lactis |
|
2.4.2.17 |
AMP |
inhibits the enzyme complex together with histidine to the T-state |
Lactococcus lactis |
|
2.4.2.17 |
histidine |
feedback inhibition, inhibits the enzyme complex together with ATP to the T-state, no inhibition of mutants E130A and Y268F/Y269F |
Lactococcus lactis |
|
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
2.4.2.17 |
Mg2+ |
dependent on |
Lactococcus lactis |
|
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
2.4.2.17 |
ATP + 5-phospho-alpha-D-ribose 1-diphosphate |
Lactococcus lactis |
first step in histidine biosynthesis |
1-(5-phospho-D-ribosyl)-ATP + diphosphate |
- |
r |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.4.2.17 |
Lactococcus lactis |
- |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
2.4.2.17 |
recombinant wild-type and selenomethionine-labeled enzyme complex comprising subunits HisGs and HisZ from Escherichia coli |
Lactococcus lactis |
Reaction
EC Number |
Reaction |
Comment |
Organism |
Reaction ID |
---|
2.4.2.17 |
1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate = ATP + 5-phospho-alpha-D-ribose 1-diphosphate |
substrate binding sites, active site structure, switch between active and inactive conformation |
Lactococcus lactis |
|
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
2.4.2.17 |
ATP + 5-phospho-alpha-D-ribose 1-diphosphate |
- |
Lactococcus lactis |
1-(5-phospho-D-ribosyl)-ATP + diphosphate |
- |
r |
|
2.4.2.17 |
ATP + 5-phospho-alpha-D-ribose 1-diphosphate |
first step in histidine biosynthesis |
Lactococcus lactis |
1-(5-phospho-D-ribosyl)-ATP + diphosphate |
- |
r |
|
2.4.2.17 |
additional information |
the enzyme comprises 4 catalytic subunits HisGs and 4 regulatory subunits HisZ with histidine as a ligand, 8 histidine binding sites at the subunit interfaces |
Lactococcus lactis |
? |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
2.4.2.17 |
More |
overall structure and monomer architecture, several motif 2 loops in both subunit types, switch structure between active and inactive conformation |
Lactococcus lactis |
2.4.2.17 |
octamer |
4 * catalytic subunit HisGs + 4 * regulatory subunit HisZ, (alpha,beta)4 |
Lactococcus lactis |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
2.4.2.17 |
ATP phosphoribosyl transferase |
- |
Lactococcus lactis |