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Literature summary extracted from

  • Mitra, K.; Rangaraj, N.; Shivaji, S.
    Novelty of the pyruvate metabolic enzyme dihydrolipoamide dehydrogenase in spermatozoa: Correlation of its localization, tyrosine phosphorylation and activity during sperm capacitation (2005), J. Biol. Chem., 280, 25743-25753.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.8.1.4 acrosome associated to the matrix, release of enzyme during acrosomal exocytosis Mesocricetus auratus
-
-
1.8.1.4 Golgi apparatus juxtanuclear localization, like Golgi, in spermatids Mesocricetus auratus 5794
-
1.8.1.4 mitochondrion in spermatocytes Mesocricetus auratus 5739
-
1.8.1.4 additional information release of enzyme during acrosomal exocytosis, extramitochondrial localization in epididymal spermatozoa in mammalia Mesocricetus auratus
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.1.4 dihydrolipoamide + NAD+ Mesocricetus auratus regulation of activity dependent on tyrosine-phosphorylation of the enzyme lipoamide + NADH
-
 r
1.8.1.4 additional information Mesocricetus auratus the enzyme is required for hyperactivation, i.e. enhanced motility, and acrosome reaction of hamster spermatozoa, the post-pyruvate metabolic enzyme shows dual involvement and regulation during sperm capacitation, control of the directionality of enzyme activity during sperm capacitation ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.4 Mesocricetus auratus
-
 male
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.8.1.4 phosphoprotein tyrosine-phosphorylated differently concerning time course in the principal piece of the flagella and the acrosome in capacitated spermatozoa, tyrosine-phosphorylation has regulatory function Mesocricetus auratus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.8.1.4 sperm flagellum
-
 Mesocricetus auratus
-
1.8.1.4 spermatid
-
 Mesocricetus auratus
-
1.8.1.4 spermatocyte
-
 Mesocricetus auratus
-
1.8.1.4 spermatozoon
-
 Mesocricetus auratus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.1.4 additional information
-
-
 Mesocricetus auratus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.4 dihydrolipoamide + NAD+
-
 Mesocricetus auratus lipoamide + NADH
-
 r
1.8.1.4 dihydrolipoamide + NAD+ regulation of activity dependent on tyrosine-phosphorylation of the enzyme Mesocricetus auratus lipoamide + NADH
-
 r
1.8.1.4 additional information the enzyme is required for hyperactivation, i.e. enhanced motility, and acrosome reaction of hamster spermatozoa, the post-pyruvate metabolic enzyme shows dual involvement and regulation during sperm capacitation, control of the directionality of enzyme activity during sperm capacitation Mesocricetus auratus ?
-
 ?
1.8.1.4 NADH + H+ + oxidized 2,6-dichlorophenolindophenol
-
 Mesocricetus auratus NAD+ + reduced 2,6-dichlorophenolindophenol
-
 ?
1.8.1.4 O2 + NADH
-
 Mesocricetus auratus H2O2 + NAD+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.8.1.4 dihydrolipoamide dehydrogenase
-
 Mesocricetus auratus
1.8.1.4 E3
-
 Mesocricetus auratus
1.8.1.4 More the enzyme is the E3 component of the pyruvate dehydrogenase multienzyme complex Mesocricetus auratus

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.4 NAD+
-
 Mesocricetus auratus
1.8.1.4 NADH
-
 Mesocricetus auratus