EC Number | Cloned (Comment) | Organism |
---|---|---|
1.8.1.8 | gene lpdA, expression in Escherichia coli strain BL21(DE3) as His-tagged protein | Mycobacterium tuberculosis |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.8.1.8 | purified recombinant His-tagged enzyme LpdA, hanging drop vapour diffusion method at room temperature, 0.003 ml protein solution containing 25 mg/ml protein, 10 mM HEPES, pH 7.5, mixed with 0.002 ml precipitant solution containing 0.1 M trisodium citrate, pH 5.4-5.8, 2.5-5.0% PEG 6000, 3-7 days depending on the pH, crystallization takes longer at higher pH-value, crystals are immersed in 10% PEG 6000, 100 mM MES, pH 5.75, and 25% glycerol, X-ray diffraction structure determination and analysis at 2.8 A resolution | Mycobacterium tuberculosis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.8.1.8 | 2'-phospho-ADP-ribose | inhibition of 2,6-dimethyl-1,4-benzoquinone reduction | Mycobacterium tuberculosis | |
1.8.1.8 | NADP+ | inhibition of 2,6-dimethyl-1,4-benzoquinone reduction | Mycobacterium tuberculosis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.1.8 | additional information | - |
additional information | steady-state kinetics, ping-pong kinetic mechanism with NAD(P)H and 2,6-dimethyl-1,4-benzoquinone or 5-hydroxy-1,4-naphthoquinone | Mycobacterium tuberculosis | |
1.8.1.8 | 0.00039 | - |
5-hydroxy-1,4-naphthoquinone | pH 7.5, 25°C, NADPH | Mycobacterium tuberculosis | |
1.8.1.8 | 0.00044 | - |
NADPH | pH 7.5, 25°C, with 5-hydroxy-1,4-naphthoquinone | Mycobacterium tuberculosis | |
1.8.1.8 | 0.00053 | - |
NADPH | pH 7.5, 25°C, with 2,6-dimethyl-1,4-benzoquinone | Mycobacterium tuberculosis | |
1.8.1.8 | 0.0016 | - |
2,6-dimethyl-1,4-benzoquinone | pH 7.5, 25°C, NADPH | Mycobacterium tuberculosis | |
1.8.1.8 | 0.0054 | - |
thio-NAD+ | pH 7.5, 25°C, with NADH | Mycobacterium tuberculosis | |
1.8.1.8 | 0.0055 | - |
5-hydroxy-1,4-naphthoquinone | pH 7.5, 25°C, NADH | Mycobacterium tuberculosis | |
1.8.1.8 | 0.011 | - |
2,6-dimethyl-1,4-benzoquinone | pH 7.5, 25°C, NADH | Mycobacterium tuberculosis | |
1.8.1.8 | 0.018 | - |
thio-NAD+ | pH 7.5, 25°C, with NADPH | Mycobacterium tuberculosis | |
1.8.1.8 | 0.028 | - |
NADH | pH 7.5, 25°C, with 5-hydroxy-1,4-naphthoquinone | Mycobacterium tuberculosis | |
1.8.1.8 | 0.036 | - |
NADH | pH 7.5, 25°C, with 2,6-dimethyl-1,4-benzoquinone | Mycobacterium tuberculosis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.8.1.8 | protein disulfide + NAD(P)H | Mycobacterium tuberculosis | - |
protein dithiol + NAD(P)+ | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.8.1.8 | Mycobacterium tuberculosis | - |
gene lpdA | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.8.1.8 | recombinant His-tagged enzyme from Escherichia coli | Mycobacterium tuberculosis |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.8.1.8 | protein-dithiol + NAD(P)+ = protein-disulfide + NAD(P)H + H+ | detailed reaction mechanism | Mycobacterium tuberculosis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.8.1.8 | 2,6-dimethyl-1,4-benzoquinone + NAD(P)H | - |
Mycobacterium tuberculosis | 2,6-dimethyl-1,4-benzoquinol + NAD(P)+ | - |
? | |
1.8.1.8 | 5-hydroxy-1,4-naphthoquinone + NAD(P)H | - |
Mycobacterium tuberculosis | 5-hydroxy-1,4-naphthoquinol + NAD(P)+ | - |
? | |
1.8.1.8 | additional information | enzyme cannot catalyze the reduction of lipoyl substrates, because it lacks one of two cysteine residues involved in dithiol-disulfide interchange with lipoyl substrates and a His-Glu pair involved in general acid catalysis, no reduction of disulfide-bonded substrates | Mycobacterium tuberculosis | ? | - |
? | |
1.8.1.8 | NAD+ + NADH | enzyme-catalyzed interconversion | Mycobacterium tuberculosis | NADH + NAD+ | - |
? | |
1.8.1.8 | protein disulfide + NAD(P)H | - |
Mycobacterium tuberculosis | protein dithiol + NAD(P)+ | - |
r | |
1.8.1.8 | thio-NAD+ + NAD(P)H | - |
Mycobacterium tuberculosis | NAD+ + thio-NAD(P)H | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.8.1.8 | tetramer | - |
Mycobacterium tuberculosis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.8.1.8 | LpdA | - |
Mycobacterium tuberculosis |
1.8.1.8 | More | enzyme is a member of the flavoprotein disulfide reductase family of enzymes | Mycobacterium tuberculosis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.8.1.8 | 25 | - |
assay at | Mycobacterium tuberculosis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.1.8 | 0.0077 | - |
2,6-dimethyl-1,4-benzoquinone | pH 7.5, 25°C, NADPH | Mycobacterium tuberculosis | |
1.8.1.8 | 0.0077 | - |
NADPH | pH 7.5, 25°C, with 2,6-dimethyl-1,4-benzoquinone | Mycobacterium tuberculosis | |
1.8.1.8 | 0.0079 | - |
5-hydroxy-1,4-naphthoquinone | pH 7.5, 25°C, NADPH | Mycobacterium tuberculosis | |
1.8.1.8 | 0.0079 | - |
NADPH | pH 7.5, 25°C, with 5-hydroxy-1,4-naphthoquinone | Mycobacterium tuberculosis | |
1.8.1.8 | 0.0087 | - |
thio-NAD+ | pH 7.5, 25°C, NAD(P)H | Mycobacterium tuberculosis | |
1.8.1.8 | 0.0087 | - |
NAD(P)H | pH 7.5, 25°C, with thio-NAD+ | Mycobacterium tuberculosis | |
1.8.1.8 | 2.5 | - |
5-hydroxy-1,4-naphthoquinone | pH 7.5, 25°C, NADH | Mycobacterium tuberculosis | |
1.8.1.8 | 2.5 | - |
NADH | pH 7.5, 25°C, with 5-hydroxy-1,4-naphthoquinone | Mycobacterium tuberculosis | |
1.8.1.8 | 2.8 | - |
2,6-dimethyl-1,4-benzoquinone | pH 7.5, 25°C, NADH | Mycobacterium tuberculosis | |
1.8.1.8 | 2.8 | - |
NADH | pH 7.5, 25°C, with 2,6-dimethyl-1,4-benzoquinone | Mycobacterium tuberculosis | |
1.8.1.8 | 3.6 | - |
thio-NAD+ | pH 7.5, 25°C, NADH | Mycobacterium tuberculosis | |
1.8.1.8 | 3.6 | - |
NADH | pH 7.5, 25°C, with thio-NAD+ | Mycobacterium tuberculosis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.8.1.8 | 7.5 | - |
assay at | Mycobacterium tuberculosis |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.8.1.8 | FAD | tightly but noncovalentyl bound, 1 molecule per subunit, reaction mechanism | Mycobacterium tuberculosis | |
1.8.1.8 | NADP+ | tightly but noncovalentyl bound, 1 molecule per subunit, physiological cofactor | Mycobacterium tuberculosis |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.1.8 | 0.000006 | - |
NADP+ | - |
Mycobacterium tuberculosis | |
1.8.1.8 | 0.0008 | - |
2'-phospho-ADP-ribose | - |
Mycobacterium tuberculosis |