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Literature summary extracted from

  • Mashhoon, N.; Carroll, M.; Pruss, C.; Eberhard, J.; Ishikawa, S.; Estabrook, R.A.; Reich, N.
    Functional characterization of Escherichia coli DNA adenine methyltransferase, a novel target for antibiotics (2004), J. Biol. Chem., 279, 52075-52081.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.1.1.72 pharmacology enzyme is a target for antibiotics and antbiotic development Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.72 overexpression as His-tagged protein in strain XL-2Blue Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.72 methylated DNA mixed-type inhibition against S-adenosyl-L-methionine, uncompetitive against unmethylated DNA Escherichia coli
2.1.1.72 S-adenosyl-L-homocysteine competitive against S-adenosyl-L-methionine, uncompetitive against DNA Escherichia coli
2.1.1.72 sinefungin enzyme-DNA-inhibitor complex formation and structure Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.72 additional information
-
 additional information steady-state kinetics, kinetic mechanism, thermodynamics Escherichia coli
2.1.1.72 0.000017
-
 adenine in DNA pH 8.0, 37°C Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.72 S-adenosyl-L-methionine + DNA adenine Escherichia coli enzyme is a critical regulator of bacterial virulence S-adenosyl-L-homocysteine + DNA 6-methylaminopurine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.72 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.72 recombinant His-tagged enzyme from strain XL-2Blue Escherichia coli

Reaction

EC Number Reaction Comment Organism Reaction ID
2.1.1.72 S-adenosyl-L-methionine + adenine in DNA = S-adenosyl-L-homocysteine + N6-methyladenine in DNA ordered bi bi mechanism with S-adenosyl-L-methionine binding first, fast methyl transfer, slow product release Escherichia coli
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.72 S-adenosyl-L-methionine + adenine in DNA
-
 Escherichia coli S-adenosyl-L-homocysteine + N6-methyladenine in DNA
-
 ?
2.1.1.72 S-adenosyl-L-methionine + DNA adenine enzyme is a critical regulator of bacterial virulence Escherichia coli S-adenosyl-L-homocysteine + DNA 6-methylaminopurine
-
 ?
2.1.1.72 S-adenosyl-L-methionine + DNA adenine DNA substrate from calf thymus, methylation of adjacent GATC sites is distributive with DNA derived from a genetic element that controls the transcription of the adjacent genes, the first methylation event is followed by enzyme release Escherichia coli S-adenosyl-L-homocysteine + DNA 6-methylaminopurine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.72 DNA adenine methyltransferase
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.72 37
-
 assay at Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.72 0.0155
-
 adenine in DNA pH 8.0, 37°C Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.72 8
-
 assay at Escherichia coli