BRENDA - Enzyme Database

Characterization of mammalian sulfiredoxin and its reactivation of hyperoxidized peroxiredoxin through reduction of cysteine sulfinic acid in the active site to cysteine

Chang, T.S.; Jeong, W.; Woo, H.A.; Lee, S.M.; Park, S.; Rhee, S.G.; J. Biol. Chem. 279, 50994-51001 (2004)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.8.98.2
expression in Escherichia coli
Homo sapiens
1.8.98.2
expression in Escherichia coli
Mus musculus
1.8.98.2
expression in Escherichia coli
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.8.98.2
0.0012
-
thioredoxin 1
-
Homo sapiens
1.8.98.2
0.03
-
ATP
-
Homo sapiens
1.8.98.2
1.8
-
GSH
-
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.8.98.2
cytosol
-
Homo sapiens
5829
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.8.98.2
peroxiredoxin-(S-hydroxy-S-oxocysteine) + ATP + 2 R-SH
Homo sapiens
Srx is largely responsible for reduction of the Cys-SO2H of peroxiredoxin in A549 human cells
peroxiredoxin-(S-hydroxycysteine) + ADP + phosphate + R-S-S-R
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.8.98.2
Homo sapiens
-
-
-
1.8.98.2
Mus musculus
-
-
-
1.8.98.2
Rattus norvegicus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.8.98.2
-
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.8.98.2
A-549 cell
-
Homo sapiens
-
1.8.98.2
HeLa cell
-
Homo sapiens
-
1.8.98.2
kidney
-
Homo sapiens
-
1.8.98.2
lung
-
Homo sapiens
-
1.8.98.2
spleen
-
Homo sapiens
-
1.8.98.2
thymus
-
Homo sapiens
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.8.98.2
additional information
no activity with CTP, UTP, dCTP, or dTTP
659421
Homo sapiens
?
-
-
-
-
1.8.98.2
peroxiredoxin-(S-hydroxy-S-oxocysteine) + ATP + 2 R-SH
-
659421
Mus musculus
peroxiredoxin-(S-hydroxycysteine) + ADP + phosphate + R-S-S-R
-
-
-
?
1.8.98.2
peroxiredoxin-(S-hydroxy-S-oxocysteine) + ATP + 2 R-SH
-
659421
Rattus norvegicus
peroxiredoxin-(S-hydroxycysteine) + ADP + phosphate + R-S-S-R
-
-
-
?
1.8.98.2
peroxiredoxin-(S-hydroxy-S-oxocysteine) + ATP + 2 R-SH
Srx is largely responsible for reduction of the Cys-SO2H of peroxiredoxin in A549 human cells
659421
Homo sapiens
peroxiredoxin-(S-hydroxycysteine) + ADP + phosphate + R-S-S-R
-
-
-
?
1.8.98.2
peroxiredoxin-(S-hydroxy-S-oxocysteine) + ATP + 2 R-SH
both glutathione and thioredoxin are potential physiological electron donors
659421
Homo sapiens
peroxiredoxin-(S-hydroxycysteine) + ADP + phosphate + R-S-S-R
-
-
-
?
1.8.98.2
peroxiredoxin-(S-hydroxy-S-oxocysteine) + ATP + GSH
-
659421
Homo sapiens
peroxiredoxin-(S-hydroxycysteine) + ADP + phosphate + GSSG
-
-
-
-
1.8.98.2
peroxiredoxin-(S-hydroxy-S-oxocysteine) + dATP + 2 R-SH
both glutathione and thioredoxin are potential physiological electron donors
659421
Homo sapiens
peroxiredoxin-(S-hydroxycysteine) + dADP + phosphate + R-S-S-R
-
-
-
?
1.8.98.2
peroxiredoxin-(S-hydroxy-S-oxocysteine) + dGTP + 2 R-SH
both glutathione and thioredoxin are potential physiological electron donors
659421
Homo sapiens
peroxiredoxin-(S-hydroxycysteine) + GDP + phosphate + R-S-S-R
-
-
-
?
1.8.98.2
peroxiredoxin-(S-hydroxy-S-oxocysteine) + GTP + 2 R-SH
both glutathione and thioredoxin are potential physiological electron donors
659421
Homo sapiens
peroxiredoxin-(S-hydroxycysteine) + GDP + phosphate + R-S-S-R
-
-
-
?
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.8.98.2
expression in Escherichia coli
Homo sapiens
1.8.98.2
expression in Escherichia coli
Mus musculus
1.8.98.2
expression in Escherichia coli
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.8.98.2
0.0012
-
thioredoxin 1
-
Homo sapiens
1.8.98.2
0.03
-
ATP
-
Homo sapiens
1.8.98.2
1.8
-
GSH
-
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.8.98.2
cytosol
-
Homo sapiens
5829
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.8.98.2
peroxiredoxin-(S-hydroxy-S-oxocysteine) + ATP + 2 R-SH
Homo sapiens
Srx is largely responsible for reduction of the Cys-SO2H of peroxiredoxin in A549 human cells
peroxiredoxin-(S-hydroxycysteine) + ADP + phosphate + R-S-S-R
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.8.98.2
-
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.8.98.2
A-549 cell
-
Homo sapiens
-
1.8.98.2
HeLa cell
-
Homo sapiens
-
1.8.98.2
kidney
-
Homo sapiens
-
1.8.98.2
lung
-
Homo sapiens
-
1.8.98.2
spleen
-
Homo sapiens
-
1.8.98.2
thymus
-
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.8.98.2
additional information
no activity with CTP, UTP, dCTP, or dTTP
659421
Homo sapiens
?
-
-
-
-
1.8.98.2
peroxiredoxin-(S-hydroxy-S-oxocysteine) + ATP + 2 R-SH
-
659421
Mus musculus
peroxiredoxin-(S-hydroxycysteine) + ADP + phosphate + R-S-S-R
-
-
-
?
1.8.98.2
peroxiredoxin-(S-hydroxy-S-oxocysteine) + ATP + 2 R-SH
-
659421
Rattus norvegicus
peroxiredoxin-(S-hydroxycysteine) + ADP + phosphate + R-S-S-R
-
-
-
?
1.8.98.2
peroxiredoxin-(S-hydroxy-S-oxocysteine) + ATP + 2 R-SH
Srx is largely responsible for reduction of the Cys-SO2H of peroxiredoxin in A549 human cells
659421
Homo sapiens
peroxiredoxin-(S-hydroxycysteine) + ADP + phosphate + R-S-S-R
-
-
-
?
1.8.98.2
peroxiredoxin-(S-hydroxy-S-oxocysteine) + ATP + 2 R-SH
both glutathione and thioredoxin are potential physiological electron donors
659421
Homo sapiens
peroxiredoxin-(S-hydroxycysteine) + ADP + phosphate + R-S-S-R
-
-
-
?
1.8.98.2
peroxiredoxin-(S-hydroxy-S-oxocysteine) + ATP + GSH
-
659421
Homo sapiens
peroxiredoxin-(S-hydroxycysteine) + ADP + phosphate + GSSG
-
-
-
-
1.8.98.2
peroxiredoxin-(S-hydroxy-S-oxocysteine) + dATP + 2 R-SH
both glutathione and thioredoxin are potential physiological electron donors
659421
Homo sapiens
peroxiredoxin-(S-hydroxycysteine) + dADP + phosphate + R-S-S-R
-
-
-
?
1.8.98.2
peroxiredoxin-(S-hydroxy-S-oxocysteine) + dGTP + 2 R-SH
both glutathione and thioredoxin are potential physiological electron donors
659421
Homo sapiens
peroxiredoxin-(S-hydroxycysteine) + GDP + phosphate + R-S-S-R
-
-
-
?
1.8.98.2
peroxiredoxin-(S-hydroxy-S-oxocysteine) + GTP + 2 R-SH
both glutathione and thioredoxin are potential physiological electron donors
659421
Homo sapiens
peroxiredoxin-(S-hydroxycysteine) + GDP + phosphate + R-S-S-R
-
-
-
?