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Literature summary extracted from

  • Mukai, T.; Kawai, S.; Mori, S.; Mikami, B.; Murata, K.
    Crystal structure of bacterial inorganic polyphosphate/ATP-glucomannokinase. Insights into kinase evolution (2004), J. Biol. Chem., 279, 50591-50600.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.63 gene GMK, expression in Escherichia coli strain BL21(DE3) Arthrobacter sp.

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.63 purified recombinant enzyme, hanging drop vapour diffusion method, cyrstallization drop on a silconized coverslip by mixing of 0.003 ml protein solution consisting of 10 mg/ml protein in 10 mM glucose, and 10 mM potassium phosphate, pH 7.0, with an equal volume of mother liquor composed of 2.0 M ammonium sulfate, 2% v/v PEG 400, and 0.1 M HEPES, pH 8.0, cyrstals are soaked in several heavy atom derivative solutions containing 1 mM of UO2Ac2, 10 mM AgNO3, 10 mM GdCl2, 1 mM HgCl2, 10 mM K2Pt(CN)4, 2 mM TmCl2, or 1 mM EuCl3 in 0.1 M Tris-HCl at different pH-values, dependent on the heavy atom salt, for 20-60 min at 20°C, X-ray diffraction structure determiination and analysis at 3.0 A resolution, modeling Arthrobacter sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.63 (phosphate)n + D-glucose Arthrobacter sp.
-
 (phosphate)n-1 + D-glucose 6-phosphate
-
 ?
2.7.1.63 (phosphate)n + D-glucose Arthrobacter sp. KM
-
 (phosphate)n-1 + D-glucose 6-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.63 Arthrobacter sp.
-
 gene GMK
-
2.7.1.63 Arthrobacter sp. KM
-
 gene GMK
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.63 recombinant enzyme from Escherichia coli in several steps Arthrobacter sp.

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.1.63 (phosphate)n + D-glucose = (phosphate)n-1 + D-glucose 6-phosphate glucose and phosphate substrate binding site structures Arthrobacter sp.
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.63 (phosphate)n + D-glucose
-
 Arthrobacter sp. (phosphate)n-1 + D-glucose 6-phosphate
-
 ?
2.7.1.63 (phosphate)n + D-glucose
-
 Arthrobacter sp. KM (phosphate)n-1 + D-glucose 6-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.63 More structure modeling of the C-terminal and N-terminal domains, and large and small domains, respectively Arthrobacter sp.

Synonyms

EC Number Synonyms Comment Organism
2.7.1.63 GMK
-
 Arthrobacter sp.
2.7.1.63 inorganic polyphosphate/ATP-glucomannokinase
-
 Arthrobacter sp.
2.7.1.63 More the enzyme belongs to the poly(P)-dependent kinase family Arthrobacter sp.
2.7.1.63 poly(P)/ATP-glucomannokinase
-
 Arthrobacter sp.