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Literature summary extracted from

  • Viggiani, A.; Siani, L.; Notomista, E.; Birolo, L.; Pucci, P.; Di Donato, A.
    The role of the conserved residues His-246, His-199, and Tyr-255 in the catalysis of catechol 2,3-dioxygenase from Pseudomonas stutzeri OX1 (2004), J. Biol. Chem., 279, 48630-48639.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.2 expressed in Escherichia coli BL21(DE3) Pseudomonas stutzeri

Protein Variants

EC Number Protein Variants Comment Organism
1.13.11.2 H199N strongly reduced activity, significant changes in pH profile Pseudomonas stutzeri
1.13.11.2 H246A strongly reduced activity, significant changes in pH profile Pseudomonas stutzeri
1.13.11.2 H246N strongly reduced activity, significant changes in pH profile Pseudomonas stutzeri
1.13.11.2 Y255F strongly reduced activity Pseudomonas stutzeri

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.2 H2O2 partially reversible by addition of Fe(NH4)2(SO4)2 Pseudomonas stutzeri

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.2 0.0015
-
 catechol wild type enzyme, pH 7.5, 25°C Pseudomonas stutzeri
1.13.11.2 0.005
-
 catechol Y255F mutant enzyme, pH 7.5, 25°C Pseudomonas stutzeri
1.13.11.2 0.434
-
 catechol H199N mutant enzyme, pH 7.5, 25°C Pseudomonas stutzeri
1.13.11.2 0.714
-
 catechol H246N mutant enzyme, pH 7.5, 25°C Pseudomonas stutzeri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.2 35000
-
 SDS-PAGE Pseudomonas stutzeri
1.13.11.2 35100
-
 calculated from deduced amino acid sequence Pseudomonas stutzeri
1.13.11.2 35110
-
 mass sprectrometry Pseudomonas stutzeri
1.13.11.2 117000
-
 gel filtration Pseudomonas stutzeri

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.2 catechol + O2 Pseudomonas stutzeri
-
 2-hydroxymuconate semialdehyde
-
 ?
1.13.11.2 catechol + O2 Pseudomonas stutzeri OX1
-
 2-hydroxymuconate semialdehyde
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.2 Pseudomonas stutzeri Q8KLV4
-
-
1.13.11.2 Pseudomonas stutzeri OX1 Q8KLV4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.2 recombinant enzyme Pseudomonas stutzeri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.2 3-methoxycatechol + O2 rapid inactivation of enzyme during turnover Pseudomonas stutzeri 2-hydroxy-3-methoxymuconate semialdehyde
-
 ?
1.13.11.2 3-methoxycatechol + O2 rapid inactivation of enzyme during turnover Pseudomonas stutzeri OX1 2-hydroxy-3-methoxymuconate semialdehyde
-
 ?
1.13.11.2 4-ethylcatechol + O2 rapid inactivation of enzyme during turnover Pseudomonas stutzeri 4-ethyl-2-hydroxymuconate semialdehyde
-
 ?
1.13.11.2 4-ethylcatechol + O2 rapid inactivation of enzyme during turnover Pseudomonas stutzeri OX1 4-ethyl-2-hydroxymuconate semialdehyde
-
 ?
1.13.11.2 4-formylcatechol + O2 rapid inactivation of enzyme during turnover Pseudomonas stutzeri 4-formyl-2-hydroxymuconate semialdehyde
-
 ?
1.13.11.2 4-formylcatechol + O2 rapid inactivation of enzyme during turnover Pseudomonas stutzeri OX1 4-formyl-2-hydroxymuconate semialdehyde
-
 ?
1.13.11.2 4-methylcatechol + O2 rapid inactivation of enzyme during turnover Pseudomonas stutzeri 2-hydroxy-4-methylmuconate semialdehyde
-
 ?
1.13.11.2 4-nitrocatechol + O2 rapid inactivation of enzyme during turnover Pseudomonas stutzeri 2-hydroxy-4-nitromuconate semialdehyde
-
 ?
1.13.11.2 catechol + O2
-
 Pseudomonas stutzeri 2-hydroxymuconate semialdehyde
-
 ?
1.13.11.2 catechol + O2 most effective substrate Pseudomonas stutzeri 2-hydroxymuconate semialdehyde
-
 ?
1.13.11.2 catechol + O2
-
 Pseudomonas stutzeri OX1 2-hydroxymuconate semialdehyde
-
 ?
1.13.11.2 catechol + O2 most effective substrate Pseudomonas stutzeri OX1 2-hydroxymuconate semialdehyde
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.2 tetramer 4 * 35000, SDS-PAGE, mass spectrometry Pseudomonas stutzeri

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.2 0.46
-
 catechol H199N mutant enzyme, pH 7.5, 25°C Pseudomonas stutzeri
1.13.11.2 1.6
-
 catechol H246N mutant enzyme, pH 7.5, 25°C Pseudomonas stutzeri
1.13.11.2 8.8
-
 catechol Y255F mutant enzyme, pH 7.5, 25°C Pseudomonas stutzeri
1.13.11.2 200
-
 catechol wild type enzyme, pH 7.5, 25°C Pseudomonas stutzeri

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.2 6
-
 rapid loss of activity below pH 6, very slow decrease of activity above pH 6 Pseudomonas stutzeri

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.13.11.2 6
-
 rapid loss of activity below pH 6 Pseudomonas stutzeri