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Literature summary extracted from

  • Hou, B.; Lim, E.K.; Higgins, G.S.; Bowles, D.J.
    N-Glucosylation of cytokinins by glycosyltransferases of Arabidopsis thaliana (2004), J. Biol. Chem., 279, 47822-47832.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.118 1-methyl-3-(2-methylpropyl)-3,7-dihydro-1H-purine-2,6-dione
-
 Arabidopsis thaliana
2.4.1.118 3-isobutyl-1-methylxanthine
-
 Arabidopsis thaliana
2.4.1.118 Olomoucine
-
 Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.118 0.04
-
 N6-benzyladenine pH 8.0, 30°C Arabidopsis thaliana
2.4.1.118 0.07
-
 kinetin pH 8.0, 30°C Arabidopsis thaliana
2.4.1.118 0.07
-
 N6-isopentenyladenine pH 8.0, 30°C Arabidopsis thaliana
2.4.1.118 0.09
-
 N6-benzyladenine pH 8.0, 30°C Arabidopsis thaliana
2.4.1.118 0.13
-
 N6-isopentenyladenine pH 8.0, 30°C Arabidopsis thaliana
2.4.1.118 0.16
-
 dihydrozeatin pH 8.0, 30°C Arabidopsis thaliana
2.4.1.118 0.21
-
 kinetin pH 8.0, 30°C Arabidopsis thaliana
2.4.1.118 0.22
-
 trans-zeatin pH 8.0, 30°C Arabidopsis thaliana
2.4.1.118 0.22
-
 dihydrozeatin pH 8.0, 30°C Arabidopsis thaliana
2.4.1.118 0.24
-
 trans-zeatin pH 8.0, 30°C Arabidopsis thaliana

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.203 Mg2+ required Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.118 Arabidopsis thaliana Q9FI99 isoform UGT76C1
-
2.4.1.118 Arabidopsis thaliana Q9FIA0 isoform UGT76C2
-
2.4.1.203 Arabidopsis thaliana Q9SK82 UGT85A1
-
2.4.1.203 Arabidopsis thaliana Q9ZQ94 UGT73C5
-
2.4.1.203 Arabidopsis thaliana Q9ZQ99 UGT73C1
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.118 dihydrozeatin + TDP-glucose
-
 Arabidopsis thaliana dihydrozeatin 7-beta-D-glucoside + TDP
-
 ?
2.4.1.118 kinetin + TDP-glucose
-
 Arabidopsis thaliana kinetin 7-beta-D-glucoside + TDP
-
 ?
2.4.1.118 additional information no substrate: adenine, guanine, trans-zeatin riboside, 3-isobutyl-1-methylxanthine, 3-methyl-7-pentylamino-pyrazolo(4,3-D)-pyrimidine, o-topolin, m-topolin, thidiazuron Arabidopsis thaliana ?
-
 ?
2.4.1.118 N6-benzyladenine + TDP-glucose
-
 Arabidopsis thaliana N6-benzyladenine 7-beta-D-glucoside + TDP
-
 ?
2.4.1.118 N6-isopentenyladenine + TDP-glucose
-
 Arabidopsis thaliana N6-isopentenyladenine 7-beta-D-glucoside + TDP
-
 ?
2.4.1.118 trans-zeatin + TDP-glucose
-
 Arabidopsis thaliana trans-zeatin 7-beta-D-glucoside + TDP
-
 ?
2.4.1.203 additional information UGT85A1, UGT73C5, and UGT73C1 recognize trans-zeatin and dihydrozeatin, which have an available hydroxyl group for glucosylation and form the O-glucosides Arabidopsis thaliana ?
-
 ?
2.4.1.203 UDP-D-glucose + trans-zeatin
-
 Arabidopsis thaliana UDP + O-beta-D-glucosyl-trans-zeatin
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.203 UGT73C1
-
 Arabidopsis thaliana
2.4.1.203 UGT73C5
-
 Arabidopsis thaliana
2.4.1.203 UGT85A1
-
 Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.203 30
-
 assay at Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.203 8
-
 assay at Arabidopsis thaliana

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.1.118 0.0016
-
 Olomoucine pH 8.0, 30°C Arabidopsis thaliana
2.4.1.118 0.0025
-
 Olomoucine pH 8.0, 30°C Arabidopsis thaliana