EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.14.99.1 | additional information | cyclooxygenase catalysis by prostaglandin H synthase-1 and -2 requires activation of the normally latent enzyme by peroxide-dependent generation of a free radical at Tyr385 (PGHS-1 numbering) in the cyclooxygenase active site. The Tyr385 radical has also been linked to self-inactivation processes that impose an ultimate limit on cyclooxygenase catalysis | Homo sapiens |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.99.1 | expression of mutant enzymes in Sf9 cells | Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.14.99.1 | N382A | mutation has little effect on the cyclooxygenase specific activity or activation efficiency but almost doubles the cyclooxygenase catalytic output before self-inactivation | Homo sapiens |
1.14.99.1 | N382D | mutation has little effect on the cyclooxygenase specific activity or activation efficiency but almost doubles the cyclooxygenase catalytic output before self-inactivation | Homo sapiens |
1.14.99.1 | N382L | mutation has little effect on the cyclooxygenase specific activity or activation efficiency but almost doubles the cyclooxygenase catalytic output before self-inactivation | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.99.1 | 0.0021 | - |
arachidonate | pH 7.2, 30°C, mutant enzyme N382L | Homo sapiens | |
1.14.99.1 | 0.0021 | - |
arachidonate | pH 7.2, 30°C, PGHS-2 wild-type enzyme | Homo sapiens | |
1.14.99.1 | 0.0022 | - |
arachidonate | pH 7.2, 30°C, mutant enzyme N383D | Homo sapiens | |
1.14.99.1 | 0.0029 | - |
arachidonate | pH 7.2, 30°C, mutant enzyme N382D | Homo sapiens | |
1.14.99.1 | 0.0037 | - |
arachidonate | pH 7.2, 30°C, mutant enzyme N383H | Homo sapiens | |
1.14.99.1 | 0.0041 | - |
arachidonate | pH 7.2, 30°C, mutant enzyme N382A | Homo sapiens | |
1.14.99.1 | 0.0083 | - |
N,N,N',N'-tetramethyl-p-phenylenediamine | pH 7.2, 30°C, mutant enzyme N383H | Homo sapiens | |
1.14.99.1 | 0.01 | - |
N,N,N',N'-tetramethyl-p-phenylenediamine | pH 7.2, 30°C, mutant enzyme N383D | Homo sapiens | |
1.14.99.1 | 0.0156 | - |
N,N,N',N'-tetramethyl-p-phenylenediamine | pH 7.2, 30°C, mutant enzyme N382D | Homo sapiens | |
1.14.99.1 | 0.0163 | - |
N,N,N',N'-tetramethyl-p-phenylenediamine | pH 7.2, 30°C, PGHS-2 wild-type enzyme | Homo sapiens | |
1.14.99.1 | 0.08 | - |
guaiacol | pH 8.0, 23°C, wild-type enzyme | Homo sapiens | |
1.14.99.1 | 0.0854 | - |
N,N,N',N'-tetramethyl-p-phenylenediamine | pH 7.2, 30°C, mutant enzyme N382L | Homo sapiens | |
1.14.99.1 | 0.29 | - |
guaiacol | pH 8.0, 23°C, mutant enzyme N382L | Homo sapiens | |
1.14.99.1 | 1.3 | - |
H2O2 | pH 8.0, 23°C, wild-type enzyme | Homo sapiens | |
1.14.99.1 | 5.5 | - |
H2O2 | pH 8.0, 23°C, mutant enzyme N382L | Homo sapiens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.99.1 | Homo sapiens | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.99.1 | arachidonate + electron donor + O2 | cyclooxygenase activity | Homo sapiens | prostaglandin H2 + oxidized electron donor + H2O | - |
? | |
1.14.99.1 | H2O2 + guaiacol | peroxidase activity | Homo sapiens | ? | - |
? | |
1.14.99.1 | H2O2 + N,N,N',N'-tetramethyl-p-phenylenediamine | peroxidase activity | Homo sapiens | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.99.1 | PGHS-2 | - |
Homo sapiens |
1.14.99.1 | prostaglandin H synthase | - |
Homo sapiens |