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Literature summary extracted from

  • Bambai, B.; Rogge, C.E.; Stec, B.; Kulmacz, R.J.
    Role of Asn-382 and Thr-383 in activation and inactivation of human prostaglandin H synthase cyclooxygenase catalysis (2004), J. Biol. Chem., 279, 4084-4092.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.99.1 additional information cyclooxygenase catalysis by prostaglandin H synthase-1 and -2 requires activation of the normally latent enzyme by peroxide-dependent generation of a free radical at Tyr385 (PGHS-1 numbering) in the cyclooxygenase active site. The Tyr385 radical has also been linked to self-inactivation processes that impose an ultimate limit on cyclooxygenase catalysis Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.99.1 expression of mutant enzymes in Sf9 cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.14.99.1 N382A mutation has little effect on the cyclooxygenase specific activity or activation efficiency but almost doubles the cyclooxygenase catalytic output before self-inactivation Homo sapiens
1.14.99.1 N382D mutation has little effect on the cyclooxygenase specific activity or activation efficiency but almost doubles the cyclooxygenase catalytic output before self-inactivation Homo sapiens
1.14.99.1 N382L mutation has little effect on the cyclooxygenase specific activity or activation efficiency but almost doubles the cyclooxygenase catalytic output before self-inactivation Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.99.1 0.0021
-
arachidonate pH 7.2, 30°C, mutant enzyme N382L Homo sapiens
1.14.99.1 0.0021
-
arachidonate pH 7.2, 30°C, PGHS-2 wild-type enzyme Homo sapiens
1.14.99.1 0.0022
-
arachidonate pH 7.2, 30°C, mutant enzyme N383D Homo sapiens
1.14.99.1 0.0029
-
arachidonate pH 7.2, 30°C, mutant enzyme N382D Homo sapiens
1.14.99.1 0.0037
-
arachidonate pH 7.2, 30°C, mutant enzyme N383H Homo sapiens
1.14.99.1 0.0041
-
arachidonate pH 7.2, 30°C, mutant enzyme N382A Homo sapiens
1.14.99.1 0.0083
-
N,N,N',N'-tetramethyl-p-phenylenediamine pH 7.2, 30°C, mutant enzyme N383H Homo sapiens
1.14.99.1 0.01
-
N,N,N',N'-tetramethyl-p-phenylenediamine pH 7.2, 30°C, mutant enzyme N383D Homo sapiens
1.14.99.1 0.0156
-
N,N,N',N'-tetramethyl-p-phenylenediamine pH 7.2, 30°C, mutant enzyme N382D Homo sapiens
1.14.99.1 0.0163
-
N,N,N',N'-tetramethyl-p-phenylenediamine pH 7.2, 30°C, PGHS-2 wild-type enzyme Homo sapiens
1.14.99.1 0.08
-
guaiacol pH 8.0, 23°C, wild-type enzyme Homo sapiens
1.14.99.1 0.0854
-
N,N,N',N'-tetramethyl-p-phenylenediamine pH 7.2, 30°C, mutant enzyme N382L Homo sapiens
1.14.99.1 0.29
-
guaiacol pH 8.0, 23°C, mutant enzyme N382L Homo sapiens
1.14.99.1 1.3
-
H2O2 pH 8.0, 23°C, wild-type enzyme Homo sapiens
1.14.99.1 5.5
-
H2O2 pH 8.0, 23°C, mutant enzyme N382L Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.14.99.1 Homo sapiens
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.99.1 arachidonate + electron donor + O2 cyclooxygenase activity Homo sapiens prostaglandin H2 + oxidized electron donor + H2O
-
?
1.14.99.1 H2O2 + guaiacol peroxidase activity Homo sapiens ?
-
?
1.14.99.1 H2O2 + N,N,N',N'-tetramethyl-p-phenylenediamine peroxidase activity Homo sapiens ?
-
?

Synonyms

EC Number Synonyms Comment Organism
1.14.99.1 PGHS-2
-
Homo sapiens
1.14.99.1 prostaglandin H synthase
-
Homo sapiens