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Literature summary extracted from

  • Singh, J.; Khan, G.A.; Kinarsky, L.; Cheng, H.; Wilken, J.; Choi, K.H.; Bedows, E.; Sherman, S.; Cheng, P.W.
    Identification of disulfide bonds among the nine core 2 N-acetylglucosaminyltransferase-M cysteines conserved in the mucin beta6-N-acetylglucosaminyltransferase family (2004), J. Biol. Chem., 279, 38969-38977.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.102 CHO cells stably transfected with a pSEcTag plasmid, This plasmid contains bC2GnT-M cDNA devoid of the 5'-sequence coding the cytoplasmic tail and transmembrane domain Bos taurus
2.4.1.148 stable expression of the enzyme, lacking the cytoplasmic tail and the transmembrane domain, in CHO cells, secretion of the fully active recombinant enzyme to the culture medium Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.148 52479
-
 x * 58000, recombinant enzyme, SDS-PAGE, x * 52479, sequence calculation Bos taurus
2.4.1.148 58000
-
 x * 58000, recombinant enzyme, SDS-PAGE, x * 52479, sequence calculation Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.102 additional information Bos taurus the enzyme catalyzes the formation of all mucin beta1,6-N-acetylglucosaminides, including core 2, core 4, and blood group I structures ?
-
 ?
2.4.1.148 additional information Bos taurus the enzyme catalyzes the formation of all mucin beta1,6-N-acetylglucosaminides, including core 2, core 4, and blood group I structures ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.102 Bos taurus
-
-
-
2.4.1.148 Bos taurus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.4.1.148 glycoprotein enzyme is N-glycosylated at one or two residues N72 and/or N108 Bos taurus

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.102
-
 Bos taurus
2.4.1.148 recombinant enzyme from CHO cell culture medium by nickel affinity chromatography Bos taurus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.4.1.148 UDP-N-acetyl-D-glucosamine + N-acetyl-beta-D-glucosaminyl-(1->3)-N-acetyl-D-galactosaminyl-R = UDP + N-acetyl-beta-D-glucosaminyl-(1->6)-[N-acetyl-beta-D-glucosaminyl-(1->3)]-N-acetyl-D-galactosaminyl-R the cystine pair pattern influences the substrate specificity of the enzyme, comparison to the enzyme from mouse Bos taurus
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.102 additional information the enzyme catalyzes the formation of all mucin beta1,6-N-acetylglucosaminides, including core 2, core 4, and blood group I structures Bos taurus ?
-
 ?
2.4.1.148 additional information the enzyme catalyzes the formation of all mucin beta1,6-N-acetylglucosaminides, including core 2, core 4, and blood group I structures Bos taurus ?
-
 ?
2.4.1.148 additional information substrate specificities of wild-type and mutant enzyme, overview Bos taurus ?
-
 ?
2.4.1.148 UDP-N-acetyl-D-glucosamine + core 1 oligosaccharide
-
 Bos taurus UDP + core 2 oligosaccharide
-
 ?
2.4.1.148 UDP-N-acetyl-D-glucosamine + core 3 oligosaccharide
-
 Bos taurus UDP + core 4 oligosaccharide
-
 ?
2.4.1.148 UDP-N-acetyl-D-glucosamine + N-acetyl-beta-D-glucosaminyl-1,3-N-acetyl-D-galactosaminyl-R
-
 Bos taurus UDP + N-acetyl-beta-D-glucosaminyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,3)-N-acetyl-D-galactosaminyl-R
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.148 ? x * 58000, recombinant enzyme, SDS-PAGE, x * 52479, sequence calculation Bos taurus
2.4.1.148 More the enzyme possesses 9 cysteine residues, 8 of which forming disulfide bonds, Cys113 remains free, determination in the recombinant enzyme, overview Bos taurus

Synonyms

EC Number Synonyms Comment Organism
2.4.1.102 bC2GnT-M
-
 Bos taurus
2.4.1.102 core 2 N-acetylglucosaminyltransferase-M
-
 Bos taurus
2.4.1.148 bC2GnT-M
-
 Bos taurus
2.4.1.148 core 2 N-acetylglucosaminyltransferase-M
-
 Bos taurus
2.4.1.148 More the enzyme belongs to the mucin beta6-N-acetylglucosaminyltransferase family Bos taurus