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Literature summary extracted from

  • Risteli, M.; Niemitalo, O.; Lankinen, H.; Juffer, A.H.; Myllyla, R.
    Characterization of collagenous peptides bound to lysyl hydroxylase isoforms (2004), J. Biol. Chem., 279, 37535-37543.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.11.4 all 3 isozymes LH1-3, LH2 in 2 splicing variants, are expressed in as His-tagged proteins in Spodoptera frugiperda Sf9 cells via baculovirus infection system with the signal peptide at the N-terminus in isozymes LH2a and b and LH3 being exchanged for the signal peptide of isozyme LH1 Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.11.4 NaCl reduces the activity of the isozymes LH1, LH2a, LH2b, and LH3 with most of the synthetic nonpeptide substrates tested, in some cases the isozyme is activated or completely inhibited, overview Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.11.4 additional information
-
 additional information Km of recombinant isozymes LH1, LH2a, LH2b, and LH3 for diverse synthetic nonpeptide substrates, overview Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.11.4 NaCl reduces the activity of the isozymes LH1, LH2a, LH2b, and LH3 with most of the synthetic nonpeptide substrates tested, in some cases the isozyme is activated or completely inhibited, overview Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.11.4 procollagen L-lysine + 2-oxoglutarate + O2 Homo sapiens
-
 procollagen 5-hydroxy-L-lysine + succinate + CO2
-
 ?
1.14.11.4 procollagen L-lysine + 2-oxoglutarate + O2 Caenorhabditis elegans collagen and other proteins with collagenous domains procollagen 5-hydroxy-L-lysine + succinate + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.4 Caenorhabditis elegans
-
 1 enzyme form LH, no isozymes
-
1.14.11.4 Homo sapiens
-
 3 isozymes LH1-3, isozyme LH2 exists in 2 splicing variants LH2a and LH2b
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.11.4 recombinant His-tagged isozymes LH1-3 from Sf9 insect cells by nickel affinity chromatography Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.4 L-lysine containing nonapeptides + 2-oxoglutarate + O2 diverse nonapeptides, synthetic substrates Homo sapiens 5-hydroxy-L-lysine containing nonapeptides + succinate + CO2
-
 ?
1.14.11.4 additional information isozymes show no strict substrate specificity concerning the synthetic peptide substrates, but preferences for sequences, analysis of the different isozymes' binding affinities for the peptides whose net charges are very important, partly the peptides bind to the active site, overview Homo sapiens ?
-
 ?
1.14.11.4 additional information the enzyme is also able to glycosylate hydroxylysyl residues and is functionally similar to the isozyme LH3 of vertebrates Caenorhabditis elegans ?
-
 ?
1.14.11.4 procollagen L-lysine + 2-oxoglutarate + O2
-
 Homo sapiens procollagen 5-hydroxy-L-lysine + succinate + CO2
-
 ?
1.14.11.4 procollagen L-lysine + 2-oxoglutarate + O2 collagen and other proteins with collagenous domains Caenorhabditis elegans procollagen 5-hydroxy-L-lysine + succinate + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.11.4 LH
-
 Homo sapiens
1.14.11.4 LH
-
 Caenorhabditis elegans
1.14.11.4 lysyl hydroxylase
-
 Homo sapiens
1.14.11.4 lysyl hydroxylase
-
 Caenorhabditis elegans