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Literature summary extracted from

  • Lubelski, J.; Mazurkiewicz, P.; van Merkerk, R.; Konings, W.N.; Driessen, A.J.M.
    ydaG and ydbA of Lactococcus lactis encode a heterodimeric ATP-binding cassette-type multidrug transporter (2004), J. Biol. Chem., 279, 34449-34455.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.6.2.2 ydaG and ydbA genes, separate expression in Lactococcus lactis NZ9000 using the nisin-inducible system Lactococcus lactis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.6.2.2 membrane
-
 Lactococcus lactis 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
7.6.2.2 63000
-
 1 * 63000 + 1 * 74000, YdaG +YdbA, a stable heterodimer, SDS-PAGE Lactococcus lactis
7.6.2.2 74000
-
 1 * 63000 + 1 * 74000, YdaG +YdbA, a stable heterodimer, SDS-PAGE Lactococcus lactis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7.6.2.2 additional information Lactococcus lactis role of the YdaG/YdbA heterodimer in drug resistance ?
-
 ?
7.6.2.2 additional information Lactococcus lactis MG1363 role of the YdaG/YdbA heterodimer in drug resistance ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
7.6.2.2 Lactococcus lactis P97046 strain IL1403
-
7.6.2.2 Lactococcus lactis MG1363 P97046 strain IL1403
-

Purification (Commentary)

EC Number Purification (Comment) Organism
7.6.2.2 co-purification of YdaG and YdbA Lactococcus lactis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.6.2.2 ATP + H2O + 2',7'-bis-(2-carboxyethyl)-5,6-carboxylfluoresceinacetoxymethyl ester/in YdaG/YdbA heterodimer Lactococcus lactis ADP + phosphate + 2',7'-bis-(2-carboxyethyl)-5,6-carboxylfluoresceinacetoxymethyl ester/out
-
 ?
7.6.2.2 ATP + H2O + 2',7'-bis-(2-carboxyethyl)-5,6-carboxylfluoresceinacetoxymethyl ester/in YdaG/YdbA heterodimer Lactococcus lactis MG1363 ADP + phosphate + 2',7'-bis-(2-carboxyethyl)-5,6-carboxylfluoresceinacetoxymethyl ester/out
-
 ?
7.6.2.2 ATP + H2O + 2'-(4-ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5'-bis-1H-benzimidazole/in Hoechst 33342, YdaG/YdbA heterodimer Lactococcus lactis ADP + phosphate + 2'-(4-ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5'-bis-1H-benzimidazole/out
-
 ?
7.6.2.2 ATP + H2O + 2'-(4-ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5'-bis-1H-benzimidazole/in Hoechst 33342, YdaG/YdbA heterodimer Lactococcus lactis MG1363 ADP + phosphate + 2'-(4-ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5'-bis-1H-benzimidazole/out
-
 ?
7.6.2.2 ATP + H2O + daunomycin/in YdaG/YdbA heterodimer Lactococcus lactis ADP + phosphate + daunomycin/out
-
 ?
7.6.2.2 ATP + H2O + daunomycin/in YdaG/YdbA heterodimer Lactococcus lactis MG1363 ADP + phosphate + daunomycin/out
-
 ?
7.6.2.2 ATP + H2O + ethidium bromide/in YdaG/YdbA heterodimer Lactococcus lactis ADP + phosphate + ethidium bromide/out
-
 ?
7.6.2.2 ATP + H2O + xenobiotic/in YdaG and YdbA are two ABC multidrug half-transporters, ATPase activity is associated with the YdaG/YdbA heterodimer only and not with the individual subunits Lactococcus lactis ADP + phosphate + xenobiotic/out
-
 ?
7.6.2.2 ATP + H2O + xenobiotic/in YdaG and YdbA are two ABC multidrug half-transporters, ATPase activity is associated with the YdaG/YdbA heterodimer only and not with the individual subunits Lactococcus lactis MG1363 ADP + phosphate + xenobiotic/out
-
 ?
7.6.2.2 additional information role of the YdaG/YdbA heterodimer in drug resistance Lactococcus lactis ?
-
 ?
7.6.2.2 additional information role of the YdaG/YdbA heterodimer in drug resistance Lactococcus lactis MG1363 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
7.6.2.2 heterodimer 1 * 63000 + 1 * 74000, YdaG +YdbA, a stable heterodimer, SDS-PAGE Lactococcus lactis

Synonyms

EC Number Synonyms Comment Organism
7.6.2.2 YdaG/YdbA heterodimeric ABC multidrug transporter Lactococcus lactis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
7.6.2.2 30
-
 assay at Lactococcus lactis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6.2.2 7.4
-
 assay at Lactococcus lactis