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Literature summary extracted from

  • Hsiao, Y.S.; Jogl, G.; Tong, L.
    Structural and biochemical studies of the substrate selectivity of carnitine acetyltransferase (2004), J. Biol. Chem., 279, 31584-31589.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.7 residues 30-626 expressed as His-tag fusion protein in Escherichia coli Mus musculus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.1.7 M564G and F565A mutant enzymes crystallized by sitting-drop vapor diffusion method Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.7 F565A used for crystallization, only minor effect on protein structure, increased activity with acetyl-CoA Mus musculus
2.3.1.7 M564A used for crystallization, decreased activity with acetyl-CoA and butyryl-CoA Mus musculus
2.3.1.7 M564G used for crystallization, dramatic changes in protein structure, decreased activity with acetyl-CoA and butyryl-CoA, strongly increased activity with hexanoyl-CoA Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.7 acetyl-CoA + carnitine Mus musculus modulates acetyl-CoA and CoA availability for a wide range of acyl-transfer reactions, involved in fatty acid metabolism CoA + O-acetylcarnitine
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.7 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.7 recombinant enzyme using His-tag Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.7 acetyl-CoA + carnitine
-
 Mus musculus CoA + O-acetylcarnitine
-
 r
2.3.1.7 acetyl-CoA + carnitine modulates acetyl-CoA and CoA availability for a wide range of acyl-transfer reactions, involved in fatty acid metabolism Mus musculus CoA + O-acetylcarnitine
-
 r
2.3.1.7 butyryl-CoA + carnitine
-
 Mus musculus CoA + O-butyrylcarnitine
-
 r

Subunits

EC Number Subunits Comment Organism
2.3.1.7 monomer wild type and mutant enzymes Mus musculus