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Literature summary extracted from

  • Kukkola, L.; Koivunen, P.; Pakkanen, O.; Page, A.P.; Myllyharju, J.
    Collagen prolyl 4-hydroxylase tetramers and dimers show identical decreases in Km values for peptide substrates with increasing chain length: mutation of one of the two catalytic sites in the tetramer inactivates the enzyme by more than half (2004), J. Biol. Chem., 279, 18656-18661.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.11.2 ascorbate required for activity Caenorhabditis elegans

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.11.2 expression of PHY-1/PHY-2/(PDI-2)2 P4H tetramer in insect cells Caenorhabditis elegans

Protein Variants

EC Number Protein Variants Comment Organism
1.14.11.2 D405 inactivation of catalytic site in PHY-2 subunit, 8% of wild-type PHY-1/PHY-2/(PDI-2)2 P4H tetramer activity Caenorhabditis elegans
1.14.11.2 D407N inactivation of catalytic site in PHY-1 subunit, 5% of wild-type PHY-1/PHY-2/(PDI-2)2 P4H tetramer activity Caenorhabditis elegans
1.14.11.2 L484A lysine of PHY-2 that binds the C-5 carboxyl group of 2-oxoglutarate, 27% of wild-type PHY-1/PHY-2/(PDI-2)2 P4H tetramer activity Caenorhabditis elegans
1.14.11.2 L486A lysine of PHY-1 that binds the C-5 carboxyl group of 2-oxoglutarate, 25% of wild-type PHY-1/PHY-2/(PDI-2)2 P4H tetramer activity Caenorhabditis elegans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.11.2 0.0002
-
 510-amino acid alpha1(I) collagen fragment PHY-1/PHY-2/(PDI-2)2 P4H tetramer Caenorhabditis elegans
1.14.11.2 0.001
-
 Fe2+
-
 Caenorhabditis elegans
1.14.11.2 0.006
-
 100-amino acid alpha1(I) collagen fragment PHY-1/PHY-2/(PDI-2)2 P4H tetramer Caenorhabditis elegans
1.14.11.2 0.025
-
 (L-Pro-L-Pro-Gly)10 PHY-1/PHY-2/(PDI-2)2 P4H tetramer Caenorhabditis elegans
1.14.11.2 0.08
-
 2-oxoglutarate
-
 Caenorhabditis elegans
1.14.11.2 0.66
-
 (L-Pro-L-Pro-Gly)5 PHY-1/PHY-2/(PDI-2)2 P4H tetramer Caenorhabditis elegans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.11.2 Fe2+ required for activity Caenorhabditis elegans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.11.2 procollagen L-proline + 2-oxoglutarate + O2 Caenorhabditis elegans
-
 procollagen trans-4-hydroxy-L-proline + succinate + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.2 Caenorhabditis elegans
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.2 (L-Pro-L-Pro-Gly)10 + 2-oxoglutarate + O2
-
 Caenorhabditis elegans (L-Pro-L-Pro-Gly)10-trans-4-hydroxy-L-proline + succinate + CO2
-
 r
1.14.11.2 (L-Pro-L-Pro-Gly)5 + 2-oxoglutarate + O2
-
 Caenorhabditis elegans (L-Pro-L-Pro-Gly)5-trans-4-hydroxy-L-proline + succinate + CO2
-
 r
1.14.11.2 (Pro-Pro-Gly)10 L-proline + 2-oxoglutarate + O2
-
 Caenorhabditis elegans (Pro-Pro-Gly)10 trans-4-hydroxy-L-proline + succinate + CO2
-
 ?
1.14.11.2 (Pro-Pro-Gly)2 L-proline + 2-oxoglutarate + O2
-
 Caenorhabditis elegans (Pro-Pro-Gly)2 trans-4-hydroxy-L-proline + succinate + CO2
-
 ?
1.14.11.2 100-amino acid alpha1(I) collagen fragment + 2-oxoglutarate + O2
-
 Caenorhabditis elegans ?
-
 r
1.14.11.2 100-amino acid alpha1(I) collagen fragment L-proline + 2-oxoglutarate + O2
-
 Caenorhabditis elegans 100-amino acid alpha1(I) collagen fragment trans-4-hydroxy-L-proline + succinate + CO2
-
 ?
1.14.11.2 510-amino acid alpha1(I) collagen fragment + 2-oxoglutarate + O2
-
 Caenorhabditis elegans ?
-
 r
1.14.11.2 510-amino acid alpha1(I) collagen fragment L-proline + 2-oxoglutarate + O2
-
 Caenorhabditis elegans 510-amino acid alpha1(I) collagen fragment trans-4-hydroxy-L-proline + succinate + CO2
-
 ?
1.14.11.2 procollagen L-proline + 2-oxoglutarate + O2
-
 Caenorhabditis elegans procollagen trans-4-hydroxy-L-proline + succinate + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.11.2 tetramer PHY-1,PHY-2,(PDI)2 subunits Caenorhabditis elegans

Synonyms

EC Number Synonyms Comment Organism
1.14.11.2 P4H
-
 Caenorhabditis elegans