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Literature summary extracted from

  • Wilson, H.L.; Rajagopalan, K.V.
    The role of tyrosine 343 in substrate binding and catalysis by human sulfite oxidase (2004), J. Biol. Chem., 279, 15105-15113.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.8.3.1 Y343F in the mutant enzyme using cytochrome c as electron acceptor, turnover number is somewhat impaired, 34% of the wild-type activity at pH 8.5. The KM-value for the mutant enzyme shows a 5fold increase over wild-type. Reduction of the molybdenum center of the Y343 F variant by sulfite is more significantly impaired at high pH than at low pH Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.3.1 0.00129
-
sulfite 25°C, pH 6.0, 20 mM buffer, wild-type enzyme Homo sapiens
1.8.3.1 0.00162
-
sulfite 25°C, pH 6.5, 20 mM buffer, wild-type enzyme Homo sapiens
1.8.3.1 0.00272
-
sulfite 25°C, pH 7.0, 20 mM buffer, wild-type enzyme Homo sapiens
1.8.3.1 0.00311
-
sulfite 25°C, pH 6.0, 20 mM buffer, mutant enzyme Y343F Homo sapiens
1.8.3.1 0.00339
-
sulfite 25°C, pH 7.5, 20 mM buffer, wild-type enzyme Homo sapiens
1.8.3.1 0.00352
-
sulfite 25°C, pH 7.0, 100 mM buffer, mutant enzyme Y343F Homo sapiens
1.8.3.1 0.00362
-
sulfite 25°C, pH 7.0, 100 mM buffer, wild-type enzyme Homo sapiens
1.8.3.1 0.00367
-
sulfite 25°C, pH 7.5, 100 mM buffer, wild-type enzyme Homo sapiens
1.8.3.1 0.00414
-
sulfite 25°C, pH 6.5, 20 mM buffer, mutant enzyme Y343F Homo sapiens
1.8.3.1 0.00423
-
sulfite 25°C, pH 7.5, 100 mM buffer, mutant enzyme Y343F Homo sapiens
1.8.3.1 0.00435
-
sulfite 25°C, pH 8.0, 20 mM buffer, wild-type enzyme Homo sapiens
1.8.3.1 0.00459
-
sulfite 25°C, pH 7.0, 20 mM buffer, mutant enzyme Y343F Homo sapiens
1.8.3.1 0.00503
-
sulfite 25°C, pH 8.25, 20 mM buffer, wild-type enzyme Homo sapiens
1.8.3.1 0.00612
-
sulfite 25°C, pH 8.0, 100 mM buffer, wild-type enzyme Homo sapiens
1.8.3.1 0.00635
-
sulfite 25°C, pH 7.5, 20 mM buffer, mutant enzyme Y343F Homo sapiens
1.8.3.1 0.00728
-
sulfite 25°C, pH 8.25, 100 mM buffer, wild-type enzyme Homo sapiens
1.8.3.1 0.00825
-
sulfite 25°C, pH 8.5, 20 mM buffer, wild-type enzyme Homo sapiens
1.8.3.1 0.00851
-
sulfite 25°C, pH 10.0, 20 mM buffer, mutant enzyme Y343F Homo sapiens
1.8.3.1 0.00859
-
sulfite 25°C, pH 8.0, 20 mM buffer, mutant enzyme Y343F Homo sapiens
1.8.3.1 0.00908
-
sulfite 25°C, pH 8.25, 20 mM buffer, mutant enzyme Y343F Homo sapiens
1.8.3.1 0.00924
-
sulfite 25°C, pH 8.5, 20 mM buffer, mutant enzyme Y343F Homo sapiens
1.8.3.1 0.00947
-
sulfite 25°C, pH 9.0, 20 mM buffer, mutant enzyme Y343F Homo sapiens
1.8.3.1 0.00959
-
sulfite 25°C, pH 8.75, 20 mM buffer, wild-type enzyme Homo sapiens
1.8.3.1 0.00963
-
sulfite 25°C, pH 9.5, 20 mM buffer, mutant enzyme Y343F Homo sapiens
1.8.3.1 0.00992
-
sulfite 25°C, pH 9.75, 20 mM buffer, mutant enzyme Y343F Homo sapiens
1.8.3.1 0.011
-
sulfite 25°C, pH 8.5, 100 mM buffer, wild-type enzyme Homo sapiens
1.8.3.1 0.0158
-
sulfite 25°C, pH 8.0, 100 mM buffer, mutant enzyme Y343F Homo sapiens
1.8.3.1 0.0221
-
sulfite 25°C, pH 9.0, 20 mM buffer, wild-type enzyme Homo sapiens
1.8.3.1 0.026
-
sulfite 25°C, pH 9.0, 100 mM buffer, wild-type enzyme Homo sapiens
1.8.3.1 0.0319
-
sulfite 25°C, pH 8.25, 100 mM buffer, mutant enzyme Y343F Homo sapiens
1.8.3.1 0.0536
-
sulfite 25°C, pH 9.5, 100 mM buffer, wild-type enzyme Homo sapiens
1.8.3.1 0.0557
-
sulfite 25°C, pH 8.5, 100 mM buffer, mutant enzyme Y343F Homo sapiens
1.8.3.1 0.0671
-
sulfite 25°C, pH 9.5, 20 mM buffer, wild-type enzyme Homo sapiens
1.8.3.1 0.147
-
sulfite 25°C, pH 9.0, 100 mM buffer, mutant enzyme Y343F Homo sapiens
1.8.3.1 0.59 1 sulfite 25°C, pH 9.5, 100 mM buffer, mutant enzyme Y343F Homo sapiens
1.8.3.1 1.54
-
sulfite 25°C, pH 10.0, 100 mM buffer, mutant enzyme Y343F Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.8.3.1 Homo sapiens
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.3.1 sulfite + O2 + H2O
-
Homo sapiens sulfate + H2O2
-
?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8.3.1 2.06
-
sulfite 25°C, pH 7.0, 100 mM buffer, mutant enzyme Y343F Homo sapiens
1.8.3.1 3.11
-
sulfite 25°C, pH 6.0, 20 mM buffer, mutant enzyme Y343F Homo sapiens
1.8.3.1 3.26
-
sulfite 25°C, pH 7.5, 100 mM buffer, mutant enzyme Y343F Homo sapiens
1.8.3.1 4.14
-
sulfite 25°C, pH 6.5, 20 mM buffer, mutant enzyme Y343F Homo sapiens
1.8.3.1 4.59
-
sulfite 25°C, pH 7.0, 20 mM buffer, mutant enzyme Y343F Homo sapiens
1.8.3.1 6.35
-
sulfite 25°C, pH 7.5, 20 mM buffer, mutant enzyme Y343F Homo sapiens
1.8.3.1 7.17
-
sulfite 25°C, pH 8.0, 100 mM buffer, mutant enzyme Y343F Homo sapiens
1.8.3.1 8.21
-
sulfite 25°C, pH 10.0, 100 mM buffer, mutant enzyme Y343F Homo sapiens
1.8.3.1 8.51
-
sulfite 25°C, pH 10.0, 20 mM buffer, mutant enzyme Y343F Homo sapiens
1.8.3.1 8.59
-
sulfite 25°C, pH 8.0, 20 mM buffer, mutant enzyme Y343F Homo sapiens
1.8.3.1 8.72
-
sulfite 25°C, pH 8.25, 100 mM buffer, mutant enzyme Y343F Homo sapiens
1.8.3.1 9.08
-
sulfite 25°C, pH 8.25, 20 mM buffer, mutant enzyme Y343F Homo sapiens
1.8.3.1 9.2
-
sulfite 25°C, pH 8.5, 100 mM buffer, mutant enzyme Y343F Homo sapiens
1.8.3.1 9.24
-
sulfite 25°C, pH 8.5, 20 mM buffer, mutant enzyme Y343F Homo sapiens
1.8.3.1 9.47
-
sulfite 25°C, pH 9.0, 20 mM buffer, mutant enzyme Y343F Homo sapiens
1.8.3.1 9.63
-
sulfite 25°C, pH 9.5, 20 mM buffer, mutant enzyme Y343F Homo sapiens
1.8.3.1 9.92
-
sulfite 25°C, pH 9.75, 20 mM buffer, mutant enzyme Y343F Homo sapiens
1.8.3.1 9.99
-
sulfite 25°C, pH 9.0, 100 mM buffer, mutant enzyme Y343F Homo sapiens
1.8.3.1 10.5
-
sulfite 25°C, pH 9.5, 100 mM buffer, mutant enzyme Y343F Homo sapiens
1.8.3.1 12.1
-
sulfite 25°C, pH 7.0, 100 mM buffer, wild-type enzyme Homo sapiens
1.8.3.1 13.2
-
sulfite 25°C, pH 6.0, 20 mM buffer, wild-type enzyme Homo sapiens
1.8.3.1 17.2
-
sulfite 25°C, pH 7.5, 100 mM buffer, wild-type enzyme Homo sapiens
1.8.3.1 17.7
-
sulfite 25°C, pH 6.5, 20 mM buffer, wild-type enzyme Homo sapiens
1.8.3.1 23.6
-
sulfite 25°C, pH 8.75, 20 mM buffer, wild-type enzyme Homo sapiens
1.8.3.1 24.2
-
sulfite 25°C, pH 7.0, 20 mM buffer, wild-type enzyme Homo sapiens
1.8.3.1 24.6
-
sulfite 25°C, pH 9.5, 100 mM buffer, wild-type enzyme Homo sapiens
1.8.3.1 24.7
-
sulfite 25°C, pH 7.5, 20 mM buffer, wild-type enzyme Homo sapiens
1.8.3.1 24.8
-
sulfite 25°C, pH 8.25, 20 mM buffer, wild-type enzyme Homo sapiens
1.8.3.1 25
-
sulfite 25°C, pH 8.0, 100 mM buffer, wild-type enzyme Homo sapiens
1.8.3.1 25.7
-
sulfite 25°C, pH 9.0, 20 mM buffer, wild-type enzyme Homo sapiens
1.8.3.1 25.9
-
sulfite 25°C, pH 8.0, 20 mM buffer, wild-type enzyme Homo sapiens
1.8.3.1 26.3
-
sulfite 25°C, pH 9.5, 20 mM buffer, wild-type enzyme Homo sapiens
1.8.3.1 26.9
-
sulfite 25°C, pH 8.5, 100 mM buffer, wild-type enzyme Homo sapiens
1.8.3.1 26.9
-
sulfite 25°C, pH 8.5, 20 mM buffer, wild-type enzyme Homo sapiens
1.8.3.1 27
-
sulfite 25°C, pH 8.25, 100 mM buffer, wild-type enzyme Homo sapiens
1.8.3.1 28.1
-
sulfite 25°C, pH 9.0, 100 mM buffer, wild-type enzyme Homo sapiens