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Literature summary extracted from

  • Morillas, M.; Gomez-Puertas, P.; Bentebibel, A.; Selles, E.; Casals, N.; Valencia, A.; Hegardt, F.G.; Asins, G.; Serra, D.
    Identification of conserved amino acid residues in rat liver carnitine palmitoyltransferase I critical for malonyl-CoA inhibition (2003), J. Biol. Chem., 278, 9058-9063.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.21 expression of carnitine palmitoyltransferase I wild-type and mutant enzymes in Saccharomyces cerevisiae Rattus norvegicus

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.21 A478G 3.2fold increase in IC50 for malonyl-CoA, 2.6fold increase in KM-value for carnitine, 3.1fold increase in Km-value for palmitoyl-CoA as compared to wild-type enzyme Rattus norvegicus
2.3.1.21 C608A 2.2fold increase in IC50 for malonyl-CoA, 2.5fold increase in KM-value for carnitine, 5fold increase in Km-value for palmitoyl-CoA as compared to wild-type enzyme Rattus norvegicus
2.3.1.21 M593A 12.6fold increase in IC50 for malonyl-CoA, 2.3fold increase in KM-value for carnitine, 1.2fold increase in Km-value for palmitoyl-CoA as compared to wild-type enzyme Rattus norvegicus
2.3.1.21 M593E 18fold increase in IC50 for malonyl-CoA, 1.2fold increase in KM-value for carnitine, 1.3fold decrease in Km-value for palmitoyl-CoA as compared to wild-type enzyme Rattus norvegicus
2.3.1.21 M593S 26fold increase in IC50 for malonyl-CoA, KM-value for carnitine is nearly identical to wild-type value, 1.5fold increase in Km-value for palmitoyl-CoA as compared to wild-type enzyme Rattus norvegicus
2.3.1.21 N464D 1.4fold decrease in IC50 for malonyl-CoA, 1.8fold increase in KM-value for carnitine, 1.2fold decrease in Km-value for palmitoyl-CoA as compared to wild-type enzyme Rattus norvegicus
2.3.1.21 T314S 1.2fold increase in IC50 for malonyl-CoA, 1.4fold increase in KM-value for carnitine, 2.9fold decrease in Km-value for palmitoyl-CoA as compared to wild-type enzyme Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.21 malonyl-CoA IC50: 0.0123 for wild-type enzyme, 0.015 mM for mutant enzyme T314S, 0.0087 mM for mutant enzyme N464D, 0.0395 mM for mutant enzyme A478G, 0.0275 mM for mutant enzyme C608A, 0.319 mM for mutant enzyme M593 mM, 0.155 mM for mutant enzyme M593A, 0.22 mM for mutant enzyme M593E Rattus norvegicus
2.3.1.137 malonyl-CoA regulatory role, enzyme belongs to the group of malonyl-CoA inhibitable carnitine acyltransferases with highly conserved residues being involved in the inhibition, i.e. Thr314, Asn464, Ala478, Met593, and Cys608, amino acid sequence comparison with enzymes from other species Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.21 0.0017
-
 palmitoyl-CoA mutant T314S Rattus norvegicus
2.3.1.21 0.0017
-
 L-carnitine mutant T314S Rattus norvegicus
2.3.1.21 0.0041
-
 palmitoyl-CoA mutant N464D Rattus norvegicus
2.3.1.21 0.0041
-
 L-carnitine mutant N464D Rattus norvegicus
2.3.1.21 0.0049
-
 palmitoyl-CoA wild-type Rattus norvegicus
2.3.1.21 0.0049
-
 L-carnitine wild-type Rattus norvegicus
2.3.1.21 0.0061
-
 palmitoyl-CoA mutant M593A Rattus norvegicus
2.3.1.21 0.0061
-
 L-carnitine mutant M593A Rattus norvegicus
2.3.1.21 0.0063
-
 palmitoyl-CoA mutant M593E Rattus norvegicus
2.3.1.21 0.0063
-
 L-carnitine mutant M593E Rattus norvegicus
2.3.1.21 0.0074
-
 palmitoyl-CoA mutant M593S Rattus norvegicus
2.3.1.21 0.0074
-
 L-carnitine mutant M593S Rattus norvegicus
2.3.1.21 0.0151
-
 palmitoyl-CoA mutant A478G Rattus norvegicus
2.3.1.21 0.0151
-
 L-carnitine mutant A478G Rattus norvegicus
2.3.1.21 0.0243
-
 palmitoyl-CoA mutant C608A Rattus norvegicus
2.3.1.21 0.0243
-
 L-carnitine mutant C608A Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.21 mitochondrion
-
 Rattus norvegicus 5739
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.137 octanoyl-CoA + L-carnitine Rattus norvegicus
-
 CoA + L-octanoylcarnitine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.21 Rattus norvegicus P32198
-
-
2.3.1.137 Rattus norvegicus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.21 liver
-
 Rattus norvegicus
-
2.3.1.137 liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.21 palmitoyl-CoA + L-carnitine
-
 Rattus norvegicus CoA + L-palmitoylcarnitine
-
 ?
2.3.1.137 octanoyl-CoA + L-carnitine
-
 Rattus norvegicus CoA + L-octanoylcarnitine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.21 carnitine palmitoyltransferase I
-
 Rattus norvegicus
2.3.1.21 CPT I
-
 Rattus norvegicus
2.3.1.137 carnitine octanoyltransferase
-
 Rattus norvegicus
2.3.1.137 COT
-
 Rattus norvegicus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.3.1.21 0.0087
-
 IC50 0.0087 mM for mutant enzyme N464D Rattus norvegicus malonyl-CoA
2.3.1.21 0.0123
-
 IC50: 0.0123 for wild-type enzyme Rattus norvegicus malonyl-CoA
2.3.1.21 0.015
-
 IC50 0.015 mM for mutant enzyme T314S Rattus norvegicus malonyl-CoA
2.3.1.21 0.0275
-
 IC50 0.0275 mM for mutant enzyme C608A Rattus norvegicus malonyl-CoA
2.3.1.21 0.0395
-
 IC50 0.0395 mM for mutant enzyme A478G Rattus norvegicus malonyl-CoA
2.3.1.21 0.155
-
 IC50 0.155 mM for mutant enzyme M593A Rattus norvegicus malonyl-CoA
2.3.1.21 0.319
-
 IC50 0.319 mM for mutant enzyme M593 mM Rattus norvegicus malonyl-CoA