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Literature summary extracted from

  • Natsch, A.; Gfeller, H.; Gygax, P.; Schmid, J.; Acuna, G.
    A specific bacterial aminoacylase cleaves odorant precursors secreted in the human axilla (2003), J. Biol. Chem., 278, 5718-5727.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.14 expressed in Escherichia coli Corynebacterium striatum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.14 0.05
-
 N2-[(benzyloxy)carbonyl]-L-glutamine pH 7 Corynebacterium striatum
3.5.1.14 0.06
-
 N-lauroyl-L-glutamine pH 7 Corynebacterium striatum
3.5.1.14 0.08
-
 N-decanoyl-L-glutamine pH 7 Corynebacterium striatum
3.5.1.14 0.2
-
 N-(E)-3-methyl-2-hexenoyl-L-glutamine pH 7 Corynebacterium striatum
3.5.1.14 0.74
-
 N-3-methyl-3-hydroxy-hexanoyl-L-glutamine pH 7 Corynebacterium striatum

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.14 Corynebacterium striatum
-
-
-
3.5.1.14 Corynebacterium striatum AX20
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.14 purified by a method that includes DEAE-Sepharose CL-6B anion exchange resin, phenyl-Sepharose hydrophobic interaction resin, Mono Q strong anion exchange column and Mono P weak anion exchange column Corynebacterium striatum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.14 N-(E)-3-methyl-2-hexenoyl-L-glutamine + H2O
-
 Corynebacterium striatum (E)-3-methyl-2-hexenoyl-glutamine + L-glutamine
-
 ?
3.5.1.14 N-(E)-3-methyl-2-hexenoyl-L-glutamine + H2O
-
 Corynebacterium striatum AX20 (E)-3-methyl-2-hexenoyl-glutamine + L-glutamine
-
 ?
3.5.1.14 N-3-methyl-3-hydroxy-hexanoyl-L-glutamine + H2O
-
 Corynebacterium striatum 3-methyl-3-hydroxy-hexanoate + L-glutamine
-
 ?
3.5.1.14 N-3-methyl-3-hydroxy-hexanoyl-L-glutamine + H2O
-
 Corynebacterium striatum AX20 3-methyl-3-hydroxy-hexanoate + L-glutamine
-
 ?
3.5.1.14 N-carbobenzyloxy-L-glutamine + H2O
-
 Corynebacterium striatum ?
-
 ?
3.5.1.14 N-carbobenzyloxy-L-glutamine + H2O
-
 Corynebacterium striatum AX20 ?
-
 ?
3.5.1.14 N-decanoyl-L-glutamine + H2O
-
 Corynebacterium striatum decanoate + L-glutamine
-
 ?
3.5.1.14 N-decanoyl-L-glutamine + H2O
-
 Corynebacterium striatum AX20 decanoate + L-glutamine
-
 ?
3.5.1.14 N-lauroyl-L-glutamine + H2O
-
 Corynebacterium striatum laurate + L-glutamine
-
 ?
3.5.1.14 N-lauroyl-L-glutamine + H2O
-
 Corynebacterium striatum AX20 laurate + L-glutamine
-
 ?