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Literature summary extracted from

  • Lai, C.Y.; Cronan, J.E.
    beta-Ketoacyl-acyl carrier protein synthase III (FabH) is essential for bacterial fatty acid synthesis (2003), J. Biol. Chem., 278, 51494-51503.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.41 additional information in-frame deletion of enzyme active site region, mutant strain retains about 10% enzymic activity and fails to grow in the absence of supplementation with exogenous long-chain fatty acids. Mutant requires only long-chain unsaturated fatty acids, no saturated fatty acids Lactococcus lactis
2.3.1.180 additional information construction of an enzyme-deficient null mutant strain CL112 by in-frame deletion of FabH active site region, the mutant strain requires unsaturated, but not saturated, long-chain fatty acids for growth, and retains about 10% of wild-type acetate incorporation and fatty acid synthesis activity leading to synthesis of branched-chain fatty acids, analysis of fatty acid content of the mutant strain, overview Lactococcus lactis ssp. lactis
2.3.1.180 additional information construction of an enzyme-deficient null mutant strain in which the gene encoding the enzyme from Salmonella enterica serovar typhimurium is inserted as a single copy or in two copies, removal of one of the two copies reduces enzyme activity, removal of the single copy of the gene leads to cell death revealing that the enzyme is absolutely essential for viability, overview Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.180 acetyl-CoA + malonyl-[acyl-carrier protein] Escherichia coli
-
 acetoacetyl-[acyl-carrier protein] + CoA + CO2
-
 ?
2.3.1.180 acetyl-CoA + malonyl-[acyl-carrier protein] Lactococcus lactis ssp. lactis the enzyme is essential for fatty acid biosynthesis, especially of short-chain fatty acids, by fatty acid synthase II initiating the elongation of fatty acids acetoacetyl-[acyl-carrier protein] + CoA + CO2
-
 ?
2.3.1.180 acetyl-CoA + malonyl-[acyl-carrier protein] Lactococcus lactis ssp. lactis IL1403 the enzyme is essential for fatty acid biosynthesis, especially of short-chain fatty acids, by fatty acid synthase II initiating the elongation of fatty acids acetoacetyl-[acyl-carrier protein] + CoA + CO2
-
 ?
2.3.1.180 additional information Lactococcus lactis ssp. lactis the enzyme prefers short-chain CoA primer substrates ?
-
 ?
2.3.1.180 additional information Lactococcus lactis ssp. lactis IL1403 the enzyme prefers short-chain CoA primer substrates ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.41 Lactococcus lactis
-
 sp. lactis IL1403
-
2.3.1.180 Escherichia coli
-
 gene fabH
-
2.3.1.180 Lactococcus lactis ssp. lactis
-
 gene fabH
-
2.3.1.180 Lactococcus lactis ssp. lactis IL1403
-
 gene fabH
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.180 additional information
-
 analysis of fatty acid content of the wild-type strain Lactococcus lactis ssp. lactis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.180 acetyl-CoA + malonyl-[acyl-carrier protein]
-
 Escherichia coli acetoacetyl-[acyl-carrier protein] + CoA + CO2
-
 ?
2.3.1.180 acetyl-CoA + malonyl-[acyl-carrier protein]
-
 Lactococcus lactis ssp. lactis acetoacetyl-[acyl-carrier protein] + CoA + CO2
-
 ?
2.3.1.180 acetyl-CoA + malonyl-[acyl-carrier protein] the enzyme is essential for fatty acid biosynthesis, especially of short-chain fatty acids, by fatty acid synthase II initiating the elongation of fatty acids Lactococcus lactis ssp. lactis acetoacetyl-[acyl-carrier protein] + CoA + CO2
-
 ?
2.3.1.180 acetyl-CoA + malonyl-[acyl-carrier protein]
-
 Lactococcus lactis ssp. lactis IL1403 acetoacetyl-[acyl-carrier protein] + CoA + CO2
-
 ?
2.3.1.180 acetyl-CoA + malonyl-[acyl-carrier protein] the enzyme is essential for fatty acid biosynthesis, especially of short-chain fatty acids, by fatty acid synthase II initiating the elongation of fatty acids Lactococcus lactis ssp. lactis IL1403 acetoacetyl-[acyl-carrier protein] + CoA + CO2
-
 ?
2.3.1.180 additional information the enzyme prefers short-chain CoA primer substrates Lactococcus lactis ssp. lactis ?
-
 ?
2.3.1.180 additional information the enzyme prefers short-chain CoA primer substrates Lactococcus lactis ssp. lactis IL1403 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.180 acetoacetyl-ACP synthase
-
 Escherichia coli
2.3.1.180 acetoacetyl-ACP synthase
-
 Lactococcus lactis ssp. lactis
2.3.1.180 beta-ketoacyl-acyl carrier protein synthase III
-
 Escherichia coli
2.3.1.180 beta-ketoacyl-acyl carrier protein synthase III
-
 Lactococcus lactis ssp. lactis
2.3.1.180 FabH
-
 Escherichia coli
2.3.1.180 FabH
-
 Lactococcus lactis ssp. lactis
2.3.1.180 KAS III
-
 Escherichia coli
2.3.1.180 KAS III
-
 Lactococcus lactis ssp. lactis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.180 37
-
 assay at Lactococcus lactis ssp. lactis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.180 7
-
 assay at Lactococcus lactis ssp. lactis