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Literature summary extracted from

  • Wada, Y.; Ohya, H.; Yamaguchi, Y.; Koizumi, N.; Sano, H.
    Preferential de novo methylation of cytosine residues in non-CpG sequences by a domains rearranged DNA methyltransferase from tobacco plants (2003), J. Biol. Chem., 278, 42386-42393.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.37 expression in Sf9 cells Nicotiana tabacum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.37 0.0000273
-
 unmethylated CpG pH 7.0 Nicotiana tabacum
2.1.1.37 0.000037
-
 hemimethylated CpG pH 7.0 Nicotiana tabacum
2.1.1.37 0.0000459
-
 CpNpN pH 7.0 Nicotiana tabacum
2.1.1.37 0.0000783
-
 CpNpG pH 7.0 Nicotiana tabacum
2.1.1.37 0.00258
-
 poly(dI-dC) pH 7.0 Nicotiana tabacum
2.1.1.37 0.00636
-
 poly(dG-dC) pH 7.0 Nicotiana tabacum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.1.37 nucleus exclusively in Nicotiana tabacum 5634
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.37 S-adenosyl-L-methionine + DNA Nicotiana tabacum NtDRM1 is a de novo cytosine methyltransferase which actively excludes CpG substrate S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.37 Nicotiana tabacum
-
 cv. Xanthi
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.37
-
 Nicotiana tabacum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.37 flower
-
 Nicotiana tabacum
-
2.1.1.37 leaf
-
 Nicotiana tabacum
-
2.1.1.37 root
-
 Nicotiana tabacum
-
2.1.1.37 stem
-
 Nicotiana tabacum
-
2.1.1.37 TBY-2 cell
-
 Nicotiana tabacum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.37 S-adenosyl-L-methionine + CpG
-
 Nicotiana tabacum S-adenosyl-L-homocysteine + CpG containing 5-methylcytosine
-
 ?
2.1.1.37 S-adenosyl-L-methionine + CpNpG
-
 Nicotiana tabacum ?
-
 ?
2.1.1.37 S-adenosyl-L-methionine + CpNpN
-
 Nicotiana tabacum ?
-
 ?
2.1.1.37 S-adenosyl-L-methionine + DNA NtDRM1 is a de novo cytosine methyltransferase which actively excludes CpG substrate Nicotiana tabacum S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
 ?
2.1.1.37 S-adenosyl-L-methionine + DNA poly(dI-dC) is a better substrate than poly(dG-dC), methylation activity toward unmethylated synthetic as well as native DNA. Activity with hemimethylated DNA is lower than that for unmethylated substrates. Methylation of about 70% of the cytosines in methylatable CpNpN and CpNpG sequences but only 10% in CpG. The enzyme non-selectively methylates any cytosines except in CpG, regardless of the adjacent nucleotide at both 5' and 3' ends Nicotiana tabacum S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
 ?
2.1.1.37 S-adenosyl-L-methionine + hemimethylated CpG
-
 Nicotiana tabacum ?
-
 ?
2.1.1.37 S-adenosyl-L-methionine + poly(dG-dC)
-
 Nicotiana tabacum ?
-
 ?
2.1.1.37 S-adenosyl-L-methionine + poly(dI-dC)
-
 Nicotiana tabacum ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.37 Nt-DRM1
-
 Nicotiana tabacum