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Literature summary extracted from

  • Okajima, T.; Xu, A.; Irvine, K.D.
    Modulation of notch-ligand binding by protein O-fucosyltransferase 1 and fringe (2003), J. Biol. Chem., 278, 42340-42345.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.222 overexpression in S2 cells Drosophila melanogaster

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.221 additional information down-regulation of enzyme by RNA interference in Notch-secreting cells inhibits both Delta-Notch and Serrate-Notch binding. Overexpression of enzyme in cultured cells increases Serrate-Notch binding but inhibits Delta-Notch binding Drosophila melanogaster

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.222 Notch + UDP-D-GlcNAc Drosophila melanogaster enzyme completely inhibits binding of Notch protein to its serrate ligands by capping of O-fucose sites, regulation of enzyme activity via expression level in correspondance to the expression level of protein O-fucosyltransferase beta-D-glucosaminyl-Notch + UDP
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.221 Drosophila melanogaster
-
-
-
2.4.1.222 Drosophila melanogaster
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.222 Notch + UDP-D-GlcNAc enzyme completely inhibits binding of Notch protein to its serrate ligands by capping of O-fucose sites, regulation of enzyme activity via expression level in correspondance to the expression level of protein O-fucosyltransferase Drosophila melanogaster beta-D-glucosaminyl-Notch + UDP
-
?
2.4.1.222 Notch + UDP-D-GlcNAc Notch protein is O-fucosylated at a serine or threonine residue in the epidermal growth factor-like domain Drosophila melanogaster beta-D-glucosaminyl-Notch + UDP
-
?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.221 OFUT1
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Drosophila melanogaster
2.4.1.222 FNG
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Drosophila melanogaster

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.222 30
-
assay at Drosophila melanogaster

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.222 7
-
assay at Drosophila melanogaster