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Literature summary extracted from

  • Malygin, E.G.; Lindstrom, W.M., Jr.; Zinoviev, V.V.; Evdokimov, A.A.; Schlagman, S.L.; Reich, N.O.; Hattman, S.
    Bacteriophage T4Dam (DNA-(adenine-N6)-methyltransferase): evidence for two distinct stages of methylation under single turnover conditions (2003), J. Biol. Chem., 278, 41749-41755.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.72 additional information
-
 additional information steady-state and pre-steady-state kinetics, single turnover methylation kinetics Tequatrovirus T4

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.72 S-adenosyl-L-methionine + DNA adenine Tequatrovirus T4 unmethylated or hemimethylated DNA S-adenosyl-L-homocysteine + DNA 6-methylaminopurine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.72 Tequatrovirus T4
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.1.1.72 S-adenosyl-L-methionine + adenine in DNA = S-adenosyl-L-homocysteine + N6-methyladenine in DNA two distinct stages of methylation under single turnover conditions Tequatrovirus T4
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.72 additional information enzyme and S-adenosyl-L-methionine form a catalytically active complex Tequatrovirus T4 ?
-
 ?
2.1.1.72 S-adenosyl-L-methionine + (CGG*CCG)73 oligodeoxynucleotide duplexes 5'-GATC/5'-GATC
-
 Tequatrovirus T4 ?
-
 ?
2.1.1.72 S-adenosyl-L-methionine + DNA adenine unmethylated or hemimethylated DNA Tequatrovirus T4 S-adenosyl-L-homocysteine + DNA 6-methylaminopurine
-
 ?
2.1.1.72 S-adenosyl-L-methionine + DNA adenine unmethylated or hemimethylated DNA, two distinct stages of methylation under single turnover conditions Tequatrovirus T4 S-adenosyl-L-homocysteine + DNA 6-methylaminopurine
-
 ?
2.1.1.72 S-adenosyl-L-methionine + oligodeoxynucleotide duplexes substrate contains one single specific recognition site as 5'-GATC/5'-GATC, or modified variants Tequatrovirus T4 S-adenosyl-L-homocysteine + DNA 6-methylaminopurine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.72 monomer enzyme and S-adenosyl-L-methionine form a catalytically active complex Tequatrovirus T4

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.72 25
-
 assay at Tequatrovirus T4

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.72 additional information
-
 additional information
-
 Tequatrovirus T4
2.1.1.72 0.21
-
 oligodeoxynucleotide duplexes 5'-GATC/5'-GATC
-
 Tequatrovirus T4

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.72 8
-
 assay at Tequatrovirus T4