EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.7.5.2 | NO | substrate inhibition at high concentratins | Pyrobaculum aerophilum |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.7.5.2 | integral to membrane | - |
Pyrobaculum aerophilum | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.7.5.2 | Iron | qNOR contains two heme iron and one nonheme iron | Pyrobaculum aerophilum |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.7.5.2 | 78800 | - |
1 * 78800, deduced from nucleotide sequence | Pyrobaculum aerophilum |
1.7.5.2 | 127000 | - |
gel filtration | Pyrobaculum aerophilum |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.7.5.2 | NO + menaquinol | Pyrobaculum aerophilum | - |
N2O + H2O + menaquinone | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.7.5.2 | Pyrobaculum aerophilum | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.7.5.2 | 1% dodecyl maltoside, CM-Sepharose, hydroxyapatite | Pyrobaculum aerophilum |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.7.5.2 | 3.28 | - |
at 54.5°C | Pyrobaculum aerophilum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.7.5.2 | NO + menaquinol | - |
Pyrobaculum aerophilum | N2O + H2O + menaquinone | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.7.5.2 | monomer | 1 * 75000-80000, SDS-PAGE, assuming that qNOR binds one dodecyl maltoside micelle of about 40000-50000 Da | Pyrobaculum aerophilum |
1.7.5.2 | monomer | 1 * 78800, deduced from nucleotide sequence | Pyrobaculum aerophilum |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.7.5.2 | qNOR | - |
Pyrobaculum aerophilum |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.7.5.2 | 93 | - |
qNOR in membranes | Pyrobaculum aerophilum |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.7.5.2 | 25 | 93 | no activity detectable below 25°C | Pyrobaculum aerophilum |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.7.5.2 | 25 | - |
no loss of activity after 24 h, qNOR in membrane | Pyrobaculum aerophilum |
1.7.5.2 | 56 | - |
50% loss of activity after 630 min, qNOR in membrane | Pyrobaculum aerophilum |
1.7.5.2 | 75 | - |
50% loss of activity after 231 min, qNOR in membrane | Pyrobaculum aerophilum |
1.7.5.2 | 100 | - |
50% loss of activity after 4 min, purified qNOR | Pyrobaculum aerophilum |
1.7.5.2 | 100 | - |
50% loss of activity after 86 min, qNOR in membrane | Pyrobaculum aerophilum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.7.5.2 | 7 | 9 | - |
Pyrobaculum aerophilum |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.7.5.2 | 4 | 10 | approx. 50% of maximal activity at pH 5.0, almost no activity at pH 10.0 | Pyrobaculum aerophilum |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.7.5.2 | 0.007 | - |
NO | 54.5°C, pH 7.0, qNOR in membrane | Pyrobaculum aerophilum | |
1.7.5.2 | 0.015 | - |
NO | 54.5°C, pH 7.0, purified qNOR | Pyrobaculum aerophilum |