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Literature summary extracted from

  • de Vries, S.; Strampraad, M.J.; Lu, S.; Moenne-Loccoz, P.; Schroder, I.
    Purification and characterization of the MQH2:NO oxidoreductase from the hyperthermophilic archaeon Pyrobaculum aerophilum (2003), J. Biol. Chem., 278, 35861-35868.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.5.2 NO substrate inhibition at high concentratins Pyrobaculum aerophilum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.7.5.2 integral to membrane
-
Pyrobaculum aerophilum
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.7.5.2 Iron qNOR contains two heme iron and one nonheme iron Pyrobaculum aerophilum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.5.2 78800
-
1 * 78800, deduced from nucleotide sequence Pyrobaculum aerophilum
1.7.5.2 127000
-
gel filtration Pyrobaculum aerophilum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.7.5.2 NO + menaquinol Pyrobaculum aerophilum
-
N2O + H2O + menaquinone
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.7.5.2 Pyrobaculum aerophilum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.5.2 1% dodecyl maltoside, CM-Sepharose, hydroxyapatite Pyrobaculum aerophilum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.5.2 3.28
-
at 54.5°C Pyrobaculum aerophilum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.5.2 NO + menaquinol
-
Pyrobaculum aerophilum N2O + H2O + menaquinone
-
?

Subunits

EC Number Subunits Comment Organism
1.7.5.2 monomer 1 * 75000-80000, SDS-PAGE, assuming that qNOR binds one dodecyl maltoside micelle of about 40000-50000 Da Pyrobaculum aerophilum
1.7.5.2 monomer 1 * 78800, deduced from nucleotide sequence Pyrobaculum aerophilum

Synonyms

EC Number Synonyms Comment Organism
1.7.5.2 qNOR
-
Pyrobaculum aerophilum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.7.5.2 93
-
qNOR in membranes Pyrobaculum aerophilum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.7.5.2 25 93 no activity detectable below 25°C Pyrobaculum aerophilum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.7.5.2 25
-
no loss of activity after 24 h, qNOR in membrane Pyrobaculum aerophilum
1.7.5.2 56
-
50% loss of activity after 630 min, qNOR in membrane Pyrobaculum aerophilum
1.7.5.2 75
-
50% loss of activity after 231 min, qNOR in membrane Pyrobaculum aerophilum
1.7.5.2 100
-
50% loss of activity after 4 min, purified qNOR Pyrobaculum aerophilum
1.7.5.2 100
-
50% loss of activity after 86 min, qNOR in membrane Pyrobaculum aerophilum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.7.5.2 7 9
-
Pyrobaculum aerophilum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.7.5.2 4 10 approx. 50% of maximal activity at pH 5.0, almost no activity at pH 10.0 Pyrobaculum aerophilum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.7.5.2 0.007
-
NO 54.5°C, pH 7.0, qNOR in membrane Pyrobaculum aerophilum
1.7.5.2 0.015
-
NO 54.5°C, pH 7.0, purified qNOR Pyrobaculum aerophilum