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Literature summary extracted from

  • Nes, W.D.; Song, Z.; Dennis, A.L.; Zhou, W.; Nam, J.; Miller, M.B.
    Biosynthesis of phytosterols. Kinetic mechanism for the enzymatic C-methylation of sterols (2003), J. Biol. Chem., 278, 34505-34516.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.142
-
 Glycine max

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.142 24(28)-methylenecycloartanol competitive with respect to S-adenosyl-L-methionine Glycine max
2.1.1.142 25-Azacycloartenol noncompetitive inhibitor versus cycloartenol, uncompetitive inhibitor versus S-adenosyl-L-methionine Glycine max
2.1.1.142 cyclolaudenol dead end inhibitor analog, competitive versus cycloartenol, uncompetitive versus S-adenosyl-L-methionine Glycine max
2.1.1.142 S-adenosyl-L-homocysteine noncompetitive with respect to S-adenosyl-L-methionine Glycine max

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.142 0.02
-
 24(28)-methylenelanosterol
-
 Glycine max
2.1.1.142 0.022
-
 24(28)-methylenecycloartanol
-
 Glycine max
2.1.1.142 0.027
-
 lanosterol
-
 Glycine max
2.1.1.142 0.03
-
 Cycloartenol
-
 Glycine max
2.1.1.142 0.03
-
 24(28)-methylenelophenol
-
 Glycine max
2.1.1.142 0.032
-
 31-norcycloartenol
-
 Glycine max
2.1.1.142 0.035
-
 Zymosterol
-
 Glycine max
2.1.1.142 0.043
-
 parkeol
-
 Glycine max
2.1.1.142 0.045
-
 24-dehydropollinstanol
-
 Glycine max

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.142 41500
-
 4 * 41500, SDS-PAGE Glycine max
2.1.1.142 161000
-
 gel filtration Glycine max

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.142 Glycine max
-
 recombinant enzyme
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.142 recombinant enzyme Glycine max

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.142 additional information
-
-
 Glycine max

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.142 S-adenosyl-L-methionine + 24(28)-methylenecycloartanol
-
 Glycine max S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.142 S-adenosyl-L-methionine + 24(28)-methylenelanosterol
-
 Glycine max S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.142 S-adenosyl-L-methionine + 24(28)-methylenelophenol
-
 Glycine max S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.142 S-adenosyl-L-methionine + 24-dehydropollinstanol
-
 Glycine max S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.142 S-adenosyl-L-methionine + 31-norcycloartenol
-
 Glycine max S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.142 S-adenosyl-L-methionine + cycloartenol successive C-methylations of the DELTA24 bond occurs at the same active center. The two methylation steps can proceed by a change in chemical mechanism resulting from differences in sterol structure, concerted versus carbocation. The kinetic mechanism remains the same during the consecutive methylation of the DELTA24 bond Glycine max S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.142 S-adenosyl-L-methionine + lanosterol
-
 Glycine max S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.142 S-adenosyl-L-methionine + parkeol
-
 Glycine max S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.142 S-adenosyl-L-methionine + zymosterol
-
 Glycine max S-adenosyl-L-homocysteine + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.142 tetramer 4 * 41500, SDS-PAGE Glycine max

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.1.1.142 0.00004
-
 25-Azacycloartenol inhibition versus cycloartenol Glycine max
2.1.1.142 0.0001
-
 25-Azacycloartenol inhibition versus S-adenosyl-L-methionine Glycine max
2.1.1.142 0.007
-
 cyclolaudenol inhibition versus cycloartenol Glycine max
2.1.1.142 0.009
-
 24(28)-methylene cycloartanol inhibition versus S-adenosyl-L-methionine Glycine max
2.1.1.142 0.009
-
 cyclolaudenol inhibition versus S-adenosyl-L-methionine Glycine max
2.1.1.142 0.047
-
 S-adenosyl-L-homocysteine inhibition versus cycloartenol Glycine max
2.1.1.142 0.056
-
 S-adenosyl-L-homocysteine inhibition versus S-adenosyl-L-methionine Glycine max
2.1.1.142 0.171
-
 24(28)-methylene cycloartanol inhibition versus cycloartenol Glycine max