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Literature summary extracted from

  • Littlejohn, T.K.; Takikawa, O.; Truscott, R.J.W.; Walker, M.J.
    Asp274 and His346 are essential for heme binding and catalytic function of human indolamine 2,3-dioxygenase (2003), J. Biol. Chem., 278, 29525-29531.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.13.11.52 gamma-interferon
-
 Homo sapiens

Application

EC Number Application Comment Organism
1.13.11.52 medicine enzyme catalyzes the first and rate-limiting step in kynurenine pathway Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.13.11.52 D274A mutant without enzyme activity, may be distal ligand or essential in maintaining the conformation of the heme pocket Homo sapiens
1.13.11.52 H16A does not act as proximal ligand Homo sapiens
1.13.11.52 H303A does not act as proximal ligand Homo sapiens
1.13.11.52 H346A mutant without enzyme activity, His346 is essential for heme binding Homo sapiens
1.13.11.52 K352A mutant with diminished heme binding ability Homo sapiens
1.13.11.52 V109A mutant maintained heme binding ability Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.52 0.0019
-
 D-tryptophan H303A mutant, 50 mM potassium phosphate buffer (pH 6.5), 20 mM ascorbic acid, 0.3 mg/ml catalase, 0.01 mM methylene blue, 0.4 mM tryptophan, 37°C, 10-60 min Homo sapiens
1.13.11.52 0.0024
-
 D-tryptophan H16A, 50 mM potassium phosphate buffer (pH 6.5), 20 mM ascorbic acid, 0.3 mg/ml catalase, 0.01 mM methylene blue, 0.4 mM tryptophan, 37°C, 10-60 min Homo sapiens
1.13.11.52 0.0044
-
 D-tryptophan K352A mutant, 50 mM potassium phosphate buffer (pH 6.5), 20 mM ascorbic acid, 0.3 mg/ml catalase, 0.01 mM methylene blue, 0.4 mM tryptophan, 37°C, 10-60 min Homo sapiens
1.13.11.52 0.005
-
 D-tryptophan wild type, 50 mM potassium phosphate buffer (pH 6.5), 20 mM ascorbic acid, 0.3 mg/ml catalase, 0.01 mM methylene blue, 0.4 mM tryptophan, 37°C, 10-60 min Homo sapiens
1.13.11.52 0.0053
-
 D-tryptophan V109A mutant, 50 mM potassium phosphate buffer (pH 6.5), 20 mM ascorbic acid, 0.3 mg/ml catalase, 0.01 mM methylene blue, 0.4 mM tryptophan, 37°C, 10-60 min Homo sapiens
1.13.11.52 0.0056
-
 L-tryptophan H16A mutant, 50 mM potassium phosphate buffer (pH 6.5), 20 mM ascorbic acid, 0.3 mg/ml catalase, 0.01 mM methylene blue, 0.4 mM tryptophan, 37°C, 10-60 min Homo sapiens
1.13.11.52 0.0089
-
 L-tryptophan V109A mutant, 50 mM potassium phosphate buffer (pH 6.5), 20 mM ascorbic acid, 0.3 mg/ml catalase, 0.01 mM methylene blue, 0.4 mM tryptophan, 37°C, 10-60 min Homo sapiens
1.13.11.52 0.0094
-
 L-tryptophan H303A mutant, 50 mM potassium phosphate buffer (pH 6.5), 20 mM ascorbic acid, 0.3 mg/ml catalase, 0.01 mM methylene blue, 0.4 mM tryptophan, 37°C, 10-60 min Homo sapiens
1.13.11.52 0.02
-
 L-tryptophan wild type, 50 mM potassium phosphate buffer (pH 6.5), 20 mM ascorbic acid, 0.3 mg/ml catalase, 0.01 mM methylene blue, 0.4 mM tryptophan, 37°C, 10-60 min Homo sapiens
1.13.11.52 0.0201
-
 L-tryptophan K352A mutant, 50 mM potassium phosphate buffer (pH 6.5), 20 mM ascorbic acid, 0.3 mg/ml catalase, 0.01 mM methylene blue, 0.4 mM tryptophan, 37°C, 10-60 min Homo sapiens
1.13.11.52 0.1
-
 5-hydroxy-L-tryptophan H303A mutant, 50 mM potassium phosphate buffer (pH 6.5), 20 mM ascorbic acid, 0.3 mg/ml catalase, 0.01 mM methylene blue, 0.4 mM tryptophan, 37°C, 10-60 min Homo sapiens
1.13.11.52 0.1
-
 5-hydroxy-L-tryptophan V109A mutant, 50 mM potassium phosphate buffer (pH 6.5), 20 mM ascorbic acid, 0.3 mg/ml catalase, 0.01 mM methylene blue, 0.4 mM tryptophan, 37°C, 10-60 min Homo sapiens
1.13.11.52 0.14
-
 5-hydroxy-L-tryptophan H16A, 50 mM potassium phosphate buffer (pH 6.5), 20 mM ascorbic acid, 0.3 mg/ml catalase, 0.01 mM methylene blue, 0.4 mM tryptophan, 37°C, 10-60 min Homo sapiens
1.13.11.52 0.17
-
 5-hydroxy-L-tryptophan K352A mutant, 50 mM potassium phosphate buffer (pH 6.5), 20 mM ascorbic acid, 0.3 mg/ml catalase, 0.01 mM methylene blue, 0.4 mM tryptophan, 37°C, 10-60 min Homo sapiens
1.13.11.52 0.4
-
 5-hydroxy-L-tryptophan wild type, 50 mM potassium phosphate buffer (pH 6.5), 20 mM ascorbic acid, 0.3 mg/ml catalase, 0.01 mM methylene blue, 0.4 mM tryptophan, 37°C, 10-60 min Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.52 45000
-
 SDS-PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.52 Homo sapiens
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.52 5-hydroxy-L-tryptophan + O2
-
 Homo sapiens N-formyl-5-hydroxy-L-kynurenine
-
 ?
1.13.11.52 D-tryptophan + O2
-
 Homo sapiens N-formyl-D-kynurenine
-
 ?
1.13.11.52 L-tryptophan + O2
-
 Homo sapiens N-formyl-L-kynurenine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.52 IDO
-
 Homo sapiens