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Literature summary extracted from

  • Thomas, C.B.; Scavetta, R.D.; Gumport, R.I.; Churchill, M.E.
    Structures of liganded and unliganded RsrI N6-adenine DNA methyltransferase: a distinct orientation for active cofactor binding (2003), J. Biol. Chem., 278, 26094-26101.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.72 expression of the apo-enzyme mutant L72P as His-tagged enzyme Cereibacter sphaeroides

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.1.72 enzyme complexed with substrate S-adenosyl-L-methionine, product S-adenosyl-L-homocysteine, or inhibitor sinefungin, and L72P mutant apo-enzyme, 2.0 mg/ml protein in crystallization buffer containing 100 mM HEPES, pH 7.4, 1.5 M LiSO4, and 1-20 mM of the ligand compounds, X-ray diffraction structure determination and analysis at 2.3 A resolution Cereibacter sphaeroides

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.72 L72P site-directed mutagenesis, altered secondary structure, the active site is pushed away from the ligand binding site, especially by altered position of Trp84 Cereibacter sphaeroides

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.72 sinefungin binding structure Cereibacter sphaeroides

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.72 S-adenosyl-L-methionine + DNA adenine Cereibacter sphaeroides
-
 S-adenosyl-L-homocysteine + DNA 6-methylaminopurine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.72 Cereibacter sphaeroides P14751
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.72 S-adenosyl-L-methionine + DNA adenine
-
 Cereibacter sphaeroides S-adenosyl-L-homocysteine + DNA 6-methylaminopurine
-
 ?
2.1.1.72 S-adenosyl-L-methionine + DNA adenine substrate and product binding site structures Cereibacter sphaeroides S-adenosyl-L-homocysteine + DNA 6-methylaminopurine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.72 M-RsrI
-
 Cereibacter sphaeroides
2.1.1.72 N6_N4_MTase
-
 Cereibacter sphaeroides
2.1.1.72 RsrI N6-adenine DNA methyltransferase
-
 Cereibacter sphaeroides