Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Shirley, A.M.; Chapple, C.
    Biochemical characterization of sinapoylglucose:choline sinapoyltransferase, a serine carboxypeptidase-like protein that functions as an acyltransferase in plant secondary metabolism (2003), J. Biol. Chem., 278, 19870-19877.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.91 expression in a Saccharomyces cerevisiae expression system that lacks the carboxypeptodase Y gene and is defective in vacuolar targeting, resulting in secretion of heterologously expressed SCT Arabidopsis sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.91 0.1
-
 1-O-caffeoyl-beta-D-glucose pH 7.0, 30°C Arabidopsis sp.
2.3.1.91 0.16
-
 1-O-sinapoyl-beta-D-glucose pH 7.0, 30°C Arabidopsis sp.
2.3.1.91 0.41
-
 1-O-feruloyl-beta-D-glucose pH 7.0, 30°C Arabidopsis sp.
2.3.1.91 1.9
-
 p-coumaroyl-beta-D-glucose pH 7.0, 30°C Arabidopsis sp.
2.3.1.91 3.2
-
 choline pH 7.0, 30°C Arabidopsis sp.
2.3.1.91 19
-
 N,N-dimethylethanolamine pH 7.0, 30°C Arabidopsis sp.
2.3.1.91 25
-
 neopentyl alcohol pH 7.0, 30°C Arabidopsis sp.
2.3.1.91 105
-
 Tris pH 7.0, 30°C Arabidopsis sp.
2.3.1.91 245
-
 2-Methylaminoethanol pH 7.0, 30°C Arabidopsis sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.91 1-O-sinapoyl-beta-D-glucose + choline Arabidopsis sp. enzyme functions in sinapate ester synthesis D-glucose + sinapoylcholine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.91 Arabidopsis sp.
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.3.1.91 proteolytic modification the 50000 Da mature protein is proteolytically processed in yeast and in planta, most likely resulting in the production of a heterodimer derived from a 30000 Da and a 17000 Da polypeptide Arabidopsis sp.

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.91
-
 Arabidopsis sp.

Storage Stability

EC Number Storage Stability Organism
2.3.1.91 -20°C, stable at -20°C glycerol for several months without loss of activity Arabidopsis sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.91 1-O-caffeoyl-beta-D-glucose + choline
-
 Arabidopsis sp. beta-D-glucose + caffeoylcholine
-
 ?
2.3.1.91 1-O-feruloyl-beta-D-glucose + choline
-
 Arabidopsis sp. beta-D-glucose + feruloylcholine
-
 ?
2.3.1.91 1-O-sinapoyl-beta-D-glucose + 2-methylaminoethanol
-
 Arabidopsis sp. beta-D-glucose + sinapoyl-2-methylaminoethanol
-
 ?
2.3.1.91 1-O-sinapoyl-beta-D-glucose + choline enzyme functions in sinapate ester synthesis Arabidopsis sp. D-glucose + sinapoylcholine
-
 ?
2.3.1.91 1-O-sinapoyl-beta-D-glucose + choline
-
 Arabidopsis sp. beta-D-glucose + sinapoylcholine
-
 ?
2.3.1.91 1-O-sinapoyl-beta-D-glucose + N,N-dimethylethanolamine
-
 Arabidopsis sp. beta-D-glucose + sinapoyl-N,N-dimethylethanolamine
-
 ?
2.3.1.91 1-O-sinapoyl-beta-D-glucose + neopentyl alcohol
-
 Arabidopsis sp. beta-D-glucose + neopentylsinapate
-
 ?
2.3.1.91 1-O-sinapoyl-beta-D-glucose + Tris
-
 Arabidopsis sp. ?
-
 ?
2.3.1.91 p-coumaroyl-beta-D-glucose + choline
-
 Arabidopsis sp. beta-D-glucose + coumaroylcholine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.91 SCT
-
 Arabidopsis sp.
2.3.1.91 sinapoylglucose:choline sinapoyltransferase
-
 Arabidopsis sp.