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Literature summary extracted from
- A role for native lipids in the stabilization and two-dimensional crystallization of the Escherichia coli NADH-ubiquinone oxidoreductase (complex I) (2003), J. Biol. Chem., 278, 19483-19491.
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 7.1.1.2 | two different two-dimensionmal crystal forms with p2 and p3 symmetry, are obtained using lipid containing native Escherichia coli extract | Escherichia coli |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 7.1.1.2 | 4°C, complex I is intact for about 2 days, stable for 3-5 days with inclusion of 10 mM CaCl2 | Escherichia coli |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 7.1.1.2 | CaCl2 | 50 mM or greater | Escherichia coli |  |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 7.1.1.2 | CaCl2 | 5-10 mM optimal for activity | Escherichia coli | |
| 7.1.1.2 | NaCl | optimal concentration between 20 mM and 50 mM | Escherichia coli | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 7.1.1.2 | 600000 | - |
gel filtration | Escherichia coli |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 7.1.1.2 | Escherichia coli | - |
Bl21 strain | - |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 7.1.1.2 | lipoprotein | native lipids play an important role in the activation, stabilization and, as a sonsequence, crystallization of purified complex I | Escherichia coli |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 7.1.1.2 | - |
Escherichia coli |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 7.1.1.2 | 57.9 | - |
- |
Escherichia coli |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 7.1.1.2 | 4°C, complex I is intact for about 2 days, stable for 3-5 days with inclusion of 10 mM CaCl2 | Escherichia coli |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 7.1.1.2 | NADH-ubiquinone oxidoreductase (complex I) | - |
Escherichia coli |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 7.1.1.2 | NADH | - |
Escherichia coli | |
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Release 2023.1 (January 2023)
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