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Literature summary extracted from

  • Huang, L.; Sexton, D.J.; Skogerson, K.; Devlin, M.; Smith, R.; Sanyal, I.; Parry, T.; Kent, R.; Enright, J.; Wu, Q.L.; Conley, G.; DeOliveira, D.; Morganelli, L.; Ducar, M.; Wescott, C.R.; Ladner, R.C.
    Novel peptide inhibitors of angiotensin-converting enzyme 2 (2003), J. Biol. Chem., 278, 15532-15540.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.17.23 expression of extracellular domain and wild-type, both as FLAG-tagged proteins, in Spodotera frugiperda Sf9 cells via baculovirus infection Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.17.23 Ac-GDYSHCSPLRYYPWWKCTYPDPEGGG-NH2 strong inhibition, most potent inhibitory peptide, i.e. DX600 Homo sapiens
3.4.17.23 additional information construction of 6 constrained peptide libraries, selected from peptide libraries displayed on phage, peptides, 21-27 amino acids, with inhibitory effects on the enzyme, specificity and stability, selection of inhibitory sequence motifs, best CXPXRXXPWXXC, overview Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.17.23 angiotensin I + H2O Homo sapiens
-
 angiotensin-(1-9) + Leu
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.17.23 Homo sapiens Q9BYF1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.17.23 recombinant wild-type and extracellular domain as FLAG-tagged proteins from Sf9 cells Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.17.23 (7-methoxycoumarin-4-yl)acetyl-YVADAPK(2,4-dinitrophenyl)-OH + H2O synthetic fluorogenic caspase-1 substrate Homo sapiens (7-methoxycoumarin-4-yl)acetyl-YVADAP + N6-(2,4-dinitrophenyl)-L-lysine
-
 ?
3.4.17.23 angiotensin I + H2O
-
 Homo sapiens angiotensin-(1-9) + Leu
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.17.23 ACE2
-
 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.17.23 8
-
 assay at Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.17.23 additional information
-
 additional information Ki values of peptides from constrained peptide libraries Homo sapiens
3.4.17.23 0.0028
-
 Ac-GDYSHCSPLRYYPWWKCTYPDPEGGG-NH2 pH 8.0, room temperature with substrate angiotensin I, pH 7.4, room temperature with substrate (7-methoxycoumarin-4-yl)acetyl-YVADAPK(2,4-dinitrophenyl)-OH Homo sapiens