Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Lu, M.L.; Huang, Y.W.; Lin, S.X.
    Purification, reconstitution, and steady-state kinetics of the trans-membrane 17beta-hydroxysteroid dehydrogenase 2 (2002), J. Biol. Chem., 277, 22123-22130.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.51 His6-tagged 17beta-HSD2 overexpressed in Sf9 cells Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.51 beta-octylglucoside strong Homo sapiens
1.1.1.51 dodecyl-beta-D-maltoside inhibition above 0.5% Homo sapiens
1.1.1.51 Triton X-100 significant Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.51 0.00025
-
 dihydrotestosterone pH 7.4, with NAD+ as cofactor Homo sapiens
1.1.1.51 0.00035
-
 estradiol pH 7.4, with NAD+ as cofactor Homo sapiens
1.1.1.51 0.00053
-
 20alpha-dihydroprogesterone pH 7.4, with NAD+ as cofactor Homo sapiens
1.1.1.51 0.00061
-
 testosterone pH 7.4, with NAD+ as cofactor Homo sapiens
1.1.1.51 0.0053
-
 NADH pH 7.4, reaction with 4-dionetestosterone Homo sapiens
1.1.1.51 0.0056
-
 NADH pH 7.4, reaction with estronel Homo sapiens
1.1.1.51 0.11
-
 NAD+ pH 7.4, reaction with estradiol Homo sapiens
1.1.1.51 0.167
-
 NAD+ pH 7.4, reaction with testosterone Homo sapiens
1.1.1.51 9.6
-
 NADP+ pH 7.4, reaction with estradiol Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.51 membrane
-
 Homo sapiens 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.51 42300
-
 2 * 42300, SDS-PAGE Homo sapiens
1.1.1.51 90400
-
 glycerol gradient ultracentrifugation Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.51 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.51 His6-tagged 17beta-HSD2 overexpressed in Sf9 cells Homo sapiens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.51 2.5
-
-
 Homo sapiens

Storage Stability

EC Number Storage Stability Organism
1.1.1.51 4°C, activity in both the homogenate and the supernatant using dodecyl-beta-D-maltoside as detergent can be kept for several days without significant loss of activity Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.51 20alpha-dihydroprogesterone + NAD+
-
 Homo sapiens 4-pregnen-3alpha,20beta-diol + NADH
-
 r
1.1.1.51 20alpha-dihydroprogesterone + NADP+
-
 Homo sapiens 4-pregnen-3alpha,20beta-diol + NADPH
-
 r
1.1.1.51 dihydrotestosterone + NAD+
-
 Homo sapiens 5alpha-androstan-3,17-dione + NADH
-
 r
1.1.1.51 dihydrotestosterone + NADP+
-
 Homo sapiens 5alpha-androstan-3,17-dione + NADPH
-
 r
1.1.1.51 estradiol + NAD+
-
 Homo sapiens estrone + NADH
-
 ?
1.1.1.51 estradiol + NADP+
-
 Homo sapiens estrone + NADPH
-
 ?
1.1.1.51 testosterone + NAD+
-
 Homo sapiens androst-4-en-3,17-dione + NADH + H+
-
 r
1.1.1.51 testosterone + NADP+
-
 Homo sapiens androst-4-en-3,17-dione + NADPH
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.51 dimer 2 * 42300, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
1.1.1.51 17beta-HSD2
-
 Homo sapiens
1.1.1.51 17beta-hydroxysteroid dehydrogenase 2
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.51 0.27
-
 NADH pH 7.4, reaction with 4-dionetestosterone Homo sapiens
1.1.1.51 0.33
-
 NADH pH 7.4, reaction with estronel Homo sapiens
1.1.1.51 0.78
-
 20alpha-dihydroprogesterone pH 7.4, with NAD+ as cofactor Homo sapiens
1.1.1.51 1.3
-
 NADP+ pH 7.4, reaction with estradiol Homo sapiens
1.1.1.51 2.4
-
 dihydrotestosterone pH 7.4, with NAD+ as cofactor Homo sapiens
1.1.1.51 2.9
-
 NAD+ pH 7.4, reaction with testosterone Homo sapiens
1.1.1.51 3.4
-
 testosterone pH 7.4, with NAD+ as cofactor Homo sapiens
1.1.1.51 3.9
-
 estradiol pH 7.4, with NAD+ as cofactor Homo sapiens
1.1.1.51 4.7
-
 NAD+ pH 7.4, reaction with estradiol Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.51 NAD+ prefers NAD+ over NADP+ Homo sapiens
1.1.1.51 NADH
-
 Homo sapiens
1.1.1.51 NADP+ prefers NAD+ over NADP+ Homo sapiens
1.1.1.51 NADPH
-
 Homo sapiens