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Literature summary extracted from

  • Ishida, H.; Noshiro, M.; Okuda, K.; Coon, M.J.
    Purification and characterization of 7alpha-hydroxy-4-cholesten-3-one 12alpha-hydroxylase (1992), J. Biol. Chem., 267, 21319-21323.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.14.139 additional information NADPH-cytochrome P-450 reductase, EC 1.6.2.4, is required for maximal activity Oryctolagus cuniculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.14.139 50000
-
 x * 50000, SDS-PAGE Oryctolagus cuniculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.139 7alpha-hydroxycholest-4-en-3-one + NADPH + H+ + O2 Oryctolagus cuniculus microsomal enzyme activity was markedly elevated by starvation or streptozotocin administration to the animals 7alpha,12alpha-dihydroxycholest-4-en-3-one + NADP+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.139 Oryctolagus cuniculus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.14.14.139 additional information immunoblotting experiment show no correlation between the enzyme activity and the amount of protein, suggesting that post-translational modification may occur Oryctolagus cuniculus

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.14.139
-
 Oryctolagus cuniculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.14.139 0.484
-
-
 Oryctolagus cuniculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.139 7alpha-hydroxycholest-4-en-3-one + NADPH + H+ + O2
-
 Oryctolagus cuniculus 7alpha,12alpha-dihydroxycholest-4-en-3-one + NADP+ + H2O
-
 ?
1.14.14.139 7alpha-hydroxycholest-4-en-3-one + NADPH + H+ + O2 microsomal enzyme activity was markedly elevated by starvation or streptozotocin administration to the animals Oryctolagus cuniculus 7alpha,12alpha-dihydroxycholest-4-en-3-one + NADP+ + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.14.139 ? x * 50000, SDS-PAGE Oryctolagus cuniculus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.139 cytochrome b5 omission of cytochrome b5 resulted in 40% loss of activity Oryctolagus cuniculus
1.14.14.139 cytochrome P450 omission results in complete loss of activity Oryctolagus cuniculus
1.14.14.139 NADPH
-
 Oryctolagus cuniculus