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Literature summary extracted from
- Purification and characterization of 7alpha-hydroxy-4-cholesten-3-one 12alpha-monooxygenase (1982), J. Biol. Chem., 257, 8030-8035.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.14.139 | additional information | NADPH-cytochrome P-450 reductase, EC 1.6.2.4, is required for maximal activity | Oryctolagus cuniculus |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.14.14.139 | stabilized at least 4 weeks under high buffer concentrations such as 300 mM phosphate buffer | Oryctolagus cuniculus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.14.139 | 5beta-cholestane-3alpha,7alpha,12alpha-triol | 0.066 mM, 62% inhibition | Oryctolagus cuniculus |  |
| 1.14.14.139 | 5beta-cholestane-3alpha,7alpha-diol | 0.066 mM, 30% inhibition | Oryctolagus cuniculus | |
| 1.14.14.139 | Emulgen 913 | 0.1% w/v, complete inhibition | Oryctolagus cuniculus | |
| 1.14.14.139 | MgCl2 | 1 mM, 10% inhibition | Oryctolagus cuniculus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.14.139 | 0.027 | - |
7alpha-hydroxycholest-4-en-3-one | - |
Oryctolagus cuniculus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.14.14.139 | microsome | - |
Oryctolagus cuniculus | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.139 | 56000 | - |
non-denaturing PAGE | Oryctolagus cuniculus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.14.139 | Oryctolagus cuniculus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.14.139 | - |
Oryctolagus cuniculus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.14.139 | liver | - |
Oryctolagus cuniculus | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.14.139 | 7alpha-hydroxycholest-4-en-3-one + NADPH + H+ + O2 | - |
Oryctolagus cuniculus | 7alpha,12alpha-dihydroxycholest-4-en-3-one + NADP+ + H2O | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.14.139 | 7alpha-hydroxy-4-cholesten-3-one 12alpha-monooxygenase | - |
Oryctolagus cuniculus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.139 | 6.7 | 7 | - |
Oryctolagus cuniculus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.139 | 6 | 7.5 | pH 6.0: about 40% of maximal activity, pH 7.5: about 65% of maximal activity | Oryctolagus cuniculus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.14.139 | cytochrome P-450 | - |
Oryctolagus cuniculus | |
| 1.14.14.139 | NADPH | - |
Oryctolagus cuniculus | |
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