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Literature summary extracted from

  • Sasaki, A.; Yamagishi, M.; Mega, T.; Norioka, S.; Natsuka, S.; Hase, S.
    Partial purification and characterization of a novel endo-beta-mannosidase acting on N-linked sugar chains from Lilium longflorum thumb (1999), J. Biochem., 125, 363-367.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.152 1.4
-
 Man-alpha-(1-6)Man-beta-(1-4)GlcNAc-beta-(1-4)GlcNAc-pyridylamine pH 5.0, 37°C Lilium longiflorum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.152 78000
-
 gel filtration Lilium longiflorum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.152 Man-beta-(1-4)GlcNAc + H2O Lilium longiflorum hydrolysis of Man-beta-(1-4)GlcNAc linkage of N-linked sugar chains, possible role in the catabolism of N-linked sugar chains leading to oligosaccharides ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.152 Lilium longiflorum
-
 Thumb
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.152 partial Lilium longiflorum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.152 flower
-
 Lilium longiflorum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.152 0.004
-
 purified enzyme, pH 5.0, 37°C Lilium longiflorum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.152 Man-alpha-(1-6)Man-beta-(1-4)GlcNAc-beta-(1-4)GlcNAc-pyridylamine + H2O
-
 Lilium longiflorum GlcNAc-beta-(1-4)GlcNAc-pyridylamine + Man-alpha-(1-6)Man
-
 ?
3.2.1.152 Man-beta-(1-4)GlcNAc + H2O hydrolysis of Man-beta-(1-4)GlcNAc linkage of N-linked sugar chains, possible role in the catabolism of N-linked sugar chains leading to oligosaccharides Lilium longiflorum ?
-
 ?
3.2.1.152 Man-beta-(1-4)GlcNAc + H2O hydrolysis of Man-beta-1-4GlcNAc linkage of N-linked sugar chains Lilium longiflorum ?
-
 ?
3.2.1.152 additional information enzyme does not hydrolyze beta-(1-4)-mannohexaose or p-nitrophenyl-beta-mannopyranoside Lilium longiflorum ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.152 endo-beta-mannosidase
-
 Lilium longiflorum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.152 5
-
-
 Lilium longiflorum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.152 additional information
-
 unstable at alkaline pH Lilium longiflorum