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Literature summary extracted from

  • Hu, C.; Jiang, P.; Xu, J.; Wu, Y.; Huang, W.
    Mutation analysis of the feedback inhibition site of phenylalanine-sensitive 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase of Escherichia coli (2003), J. Basic Microbiol., 43, 399-406.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.54 F144A inhibition by phenylalanine, 30% residual activity Escherichia coli
2.5.1.54 F209A inhibition by phenylalanine, 79% residual activity Escherichia coli
2.5.1.54 I10A inhibition by phenylalanine, 95% residual activity Escherichia coli
2.5.1.54 L175A inhibition by phenylalanine, 18% residual activity Escherichia coli
2.5.1.54 L175D inhibition by phenylalanine, 83% residual activity Escherichia coli
2.5.1.54 L175Q inhibition by phenylalanine, 44% residual activity Escherichia coli
2.5.1.54 L179A inhibition by phenylalanine, 82% residual activity Escherichia coli
2.5.1.54 additional information N-terminal deletion mutant, no inhibition by phenylalanine Escherichia coli
2.5.1.54 N5K inhibition by phenylalanine, 33% residual activity Escherichia coli
2.5.1.54 P150L inhibition by phenylalanine, no inhibition by phenylalanine Escherichia coli
2.5.1.54 V221A inhibition by phenylalanine, 95% residual activity Escherichia coli
2.5.1.54 W215A inhibition by phenylalanine, 58% residual activity Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.54 phenylalanine 1 Mn, wild-type 8.2% residual activity Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.54 Escherichia coli
-
 isoform AroG
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.54 0.29
-
 N-terminal deletion mutant, 37°C, pH 6.4 Escherichia coli
2.5.1.54 0.81
-
 mutant W215A, 37°C, pH 6.4 Escherichia coli
2.5.1.54 0.92
-
 mutant I10A, 37°C, pH 6.4 Escherichia coli
2.5.1.54 1.97
-
 mutant N5K, 37°C, pH 6.4 Escherichia coli
2.5.1.54 2.37
-
 mutant V221A, 37°C, pH 6.4 Escherichia coli
2.5.1.54 2.7
-
 wild-type, 37°C, pH 6.4 Escherichia coli
2.5.1.54 2.81
-
 mutant L179A, 37°C, pH 6.4 Escherichia coli
2.5.1.54 2.81
-
 mutant P150L, 37°C, pH 6.4 Escherichia coli
2.5.1.54 3.07
-
 mutant F144A, 37°C, pH 6.4 Escherichia coli
2.5.1.54 3.25
-
 mutant F209A, 37°C, pH 6.4 Escherichia coli
2.5.1.54 3.6
-
 mutant L175Q, 37°C, pH 6.4 Escherichia coli
2.5.1.54 4.25
-
 mutant L175A, 37°C, pH 6.4 Escherichia coli
2.5.1.54 4.46
-
 mutant L175D, 37°C, pH 6.4 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
 Escherichia coli 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate
-
 ?