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Literature summary extracted from

  • van Beilen, J.B.; Smits, T.H.; Roos, F.F.; Brunner, T.; Balada, S.B.; Rothlisberger, M.; Witholt, B.
    Identification of an amino acid position that determines the substrate range of integral membrane alkane hydroxylases (2005), J. Bacteriol., 187, 85-91.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.15.3
-
 Alcanivorax borkumensis
1.14.15.3 expressed in Pseudomonas fluorescence KOB2delta1 Pseudomonas putida
1.14.15.3 expressed in Pseudomonas fluorescence KOB2delta1 Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
1.14.15.3 H273A no activity Pseudomonas putida
1.14.15.3 H273Q no activity Pseudomonas putida
1.14.15.3 L69F oxidizes only C10 and C11 alkanes Mycobacterium tuberculosis
1.14.15.3 L69W oxidizes only C10 and C11 alkanes Mycobacterium tuberculosis
1.14.15.3 W55C prefers C11-C13 alkanes as growth substrates Pseudomonas putida
1.14.15.3 W55S prefers C11-C13 alkanes as growth substrates Pseudomonas putida

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.15.3 membrane
-
 Pseudomonas putida 16020
-
1.14.15.3 membrane
-
 Mycobacterium tuberculosis 16020
-
1.14.15.3 membrane
-
 Alcanivorax borkumensis 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.15.3 n-alkane + NAD(P)H + H+ + O2 Pseudomonas putida
-
 n-alkanol + NAD(P)+ + H2O
-
 ?
1.14.15.3 n-alkane + NAD(P)H + H+ + O2 Mycobacterium tuberculosis
-
 n-alkanol + NAD(P)+ + H2O
-
 ?
1.14.15.3 n-alkane + NAD(P)H + H+ + O2 Alcanivorax borkumensis
-
 n-alkanol + NAD(P)+ + H2O
-
 ?
1.14.15.3 n-alkane + NAD(P)H + H+ + O2 Pseudomonas putida GPo1 / ATCC29347
-
 n-alkanol + NAD(P)+ + H2O
-
 ?
1.14.15.3 n-alkane + NAD(P)H + H+ + O2 Mycobacterium tuberculosis H37Rv
-
 n-alkanol + NAD(P)+ + H2O
-
 ?
1.14.15.3 n-alkane + NAD(P)H + H+ + O2 Alcanivorax borkumensis AP1
-
 n-alkanol + NAD(P)+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.3 Alcanivorax borkumensis
-
-
-
1.14.15.3 Alcanivorax borkumensis AP1
-
-
-
1.14.15.3 Mycobacterium tuberculosis
-
-
-
1.14.15.3 Mycobacterium tuberculosis H37Rv
-
-
-
1.14.15.3 Pseudomonas putida
-
-
-
1.14.15.3 Pseudomonas putida GPo1 / ATCC29347
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.3 n-alkane + NAD(P)H + H+ + O2
-
 Pseudomonas putida n-alkanol + NAD(P)+ + H2O
-
 ?
1.14.15.3 n-alkane + NAD(P)H + H+ + O2
-
 Mycobacterium tuberculosis n-alkanol + NAD(P)+ + H2O
-
 ?
1.14.15.3 n-alkane + NAD(P)H + H+ + O2
-
 Alcanivorax borkumensis n-alkanol + NAD(P)+ + H2O
-
 ?
1.14.15.3 n-alkane + NAD(P)H + H+ + O2 native strain oxidizes C6-C13 alkanes Pseudomonas putida n-alkanol + NAD(P)+ + H2O
-
 ?
1.14.15.3 n-alkane + NAD(P)H + H+ + O2 oxidizes C10-C16 alkanes Mycobacterium tuberculosis n-alkanol + NAD(P)+ + H2O
-
 ?
1.14.15.3 n-alkane + NAD(P)H + H+ + O2
-
 Pseudomonas putida GPo1 / ATCC29347 n-alkanol + NAD(P)+ + H2O
-
 ?
1.14.15.3 n-alkane + NAD(P)H + H+ + O2 native strain oxidizes C6-C13 alkanes Pseudomonas putida GPo1 / ATCC29347 n-alkanol + NAD(P)+ + H2O
-
 ?
1.14.15.3 n-alkane + NAD(P)H + H+ + O2
-
 Mycobacterium tuberculosis H37Rv n-alkanol + NAD(P)+ + H2O
-
 ?
1.14.15.3 n-alkane + NAD(P)H + H+ + O2 oxidizes C10-C16 alkanes Mycobacterium tuberculosis H37Rv n-alkanol + NAD(P)+ + H2O
-
 ?
1.14.15.3 n-alkane + NAD(P)H + H+ + O2
-
 Alcanivorax borkumensis AP1 n-alkanol + NAD(P)+ + H2O
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.15.3 NAD(P)H
-
 Pseudomonas putida
1.14.15.3 NAD(P)H
-
 Mycobacterium tuberculosis
1.14.15.3 NAD(P)H
-
 Alcanivorax borkumensis