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Literature summary extracted from

  • Grose, J.H.; Bergthorsson, U.; Roth, J.R.
    Regulation of NAD synthesis by the trifunctional NadR protein of Salmonella enterica (2005), J. Bacteriol., 187, 2774-2782.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.22 gene nadR, expression of N-terminally His6-tagged enzyme in Escherichia coli Salmonella enterica

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.22 additional information analysis of mutant strains for activities/functions of enzyme domains, reduced activity of class I mutants, overview Salmonella enterica

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.22 NAD+ 69% inhibition at 1.2 mM, complete inhibition at 5 mM Salmonella enterica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.22 0.08
-
 N-ribosylnicotinamide recombinant enzyme, pH 7.6, 37°C Salmonella enterica
2.7.1.22 1.2
-
 ATP recombinant enzyme, pH 7.6, 37°C Salmonella enterica

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.22 Mg2+ required Salmonella enterica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.22 ATP + N-ribosylnicotinamide Salmonella enterica activity is essential for N-ribosylnicotinamide assimilation ADP + nicotinamide ribonucleotide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.22 Salmonella enterica
-
 diverse strains with different genotypes, gene nadR
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.22 recombinant N-terminally His6-tagged enzyme from Escherichia coli by nickel affinity chromatography Salmonella enterica

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.1.22 ATP + 1-(beta-D-ribofuranosyl)-nicotinamide = ADP + beta-nicotinamide D-ribonucleotide bifunctional enzyme exhibiting nicotinamide ribose kinase activity, EC 2.7.1.22, and weak adenylyltransferase activity, EC 2.7.7.1 Salmonella enterica
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.22 1.4
-
 purified recombinant enzyme Salmonella enterica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.22 ATP + N-ribosylnicotinamide
-
 Salmonella enterica ADP + nicotinamide ribonucleotide
-
 ?
2.7.1.22 ATP + N-ribosylnicotinamide activity is essential for N-ribosylnicotinamide assimilation Salmonella enterica ADP + nicotinamide ribonucleotide
-
 ?
2.7.1.22 additional information the enzymes' C-terminal domain has nicotinamide ribose kinase activity, the central domain performes adenylyltransferase reaction, EC 2.7.7.1, overview, the purified enzyme binds nadB promotor sequence in absence of ATP with or without NAD+, DNA binding activity of the enzyme is regulated by ATP binding to the adenylyltransferase domain Salmonella enterica ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.22 NadR
-
 Salmonella enterica
2.7.1.22 nicotinamide ribose kinase
-
 Salmonella enterica
2.7.1.22 NmR-K
-
 Salmonella enterica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.22 37
-
 assay at Salmonella enterica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.22 7.6
-
 assay at Salmonella enterica

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.22 ATP
-
 Salmonella enterica