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Literature summary extracted from

  • Kuhner, S.; Wohlbrand, L.; Fritz, I.; Wruck, W.; Hultschig, C.; Hufnagel, P.; Kube, M.; Reinhardt, R.; Rabus, R.
    Substrate-dependent regulation of anaerobic degradation pathways for toluene and ethylbenzene in a denitrifying bacterium, strain EbN1 (2005), J. Bacteriol., 187, 1493-1503.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.17.99.2 DNA microarray uncultured bacterium

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.17.99.2 periplasm
-
 uncultured bacterium
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.17.99.2 ethylbenzene + H2O + acceptor uncultured bacterium initial step of the degradation pathway from the toxic ethylbenzene to benzoyl-CoA (S)-1-phenylethanol + reduced acceptor
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.17.99.2 uncultured bacterium
-
 Azoarcus-like strain EbN1, denitrifying bacterium, grown with ethylbenzne as sole substrate
-
6.4.1.8 Aromatoleum aromaticum
-
-
-
6.4.1.8 Aromatoleum aromaticum EbN1
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.99.2 ethylbenzene + H2O + acceptor initial step of the degradation pathway from the toxic ethylbenzene to benzoyl-CoA uncultured bacterium (S)-1-phenylethanol + reduced acceptor
-
 ?
6.4.1.8 2 ATP + acetophenone + HCO3- + H2O + H+
-
 Aromatoleum aromaticum 2 ADP + 2 phosphate + 3-oxo-3-phenylpropanoate
-
 ?
6.4.1.8 2 ATP + acetophenone + HCO3- + H2O + H+
-
 Aromatoleum aromaticum EbN1 2 ADP + 2 phosphate + 3-oxo-3-phenylpropanoate
-
 ?

Subunits

EC Number Subunits Comment Organism
1.17.99.2 trimer alpha, beta, gamma uncultured bacterium

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.99.2 Molybdenum
-
 uncultured bacterium

Expression

EC Number Organism Comment Expression
6.4.1.8 Aromatoleum aromaticum acetophenone carboxylase is formed in ethylbenzene- and toluene-adapted cells up