EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.3.1.20 | ammonium sulfate | 45 mM, 10% increase in activity | Acinetobacter baylyi ADP1 | |
2.3.1.75 | ammonium sulfate | 45 mM, 10% increase in activtiy | Acinetobacter baylyi ADP1 |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.1.22 | gene atfA, expression of the soluble enzyme in Escherichia coli strain BL21(DE3) | Acinetobacter sp. |
EC Number | General Stability | Organism |
---|---|---|
2.3.1.20 | bovine serum albumin stabilizes, decrease in activity with buffers such as bis-Tris, HEPES, morpholinoethanesulfonic acid | Acinetobacter baylyi ADP1 |
2.3.1.75 | decrease in activity with buffers such as Tris, Bis-Tris, HEPES, morpholinoethanesulfonic acid | Acinetobacter baylyi ADP1 |
2.3.1.75 | highly sensitive to proteases, especially to metallorpoteases, proteolysis is inhibited by addition of a cocktail of protease inhibitors including EDTA | Acinetobacter baylyi ADP1 |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.3.1.20 | CaCl2 | 5 mM, 30% inhibition | Acinetobacter baylyi ADP1 | |
2.3.1.20 | CoA | above 50 mM, about 70% inhibition above 300 mM | Acinetobacter baylyi ADP1 | |
2.3.1.20 | MgCl2 | 5 mM, 17% inhibition | Acinetobacter baylyi ADP1 | |
2.3.1.20 | MnCl2 | 5 mM, 30% inhibition | Acinetobacter baylyi ADP1 | |
2.3.1.75 | CaCl2 | 5 mM, 30% inhibition | Acinetobacter baylyi ADP1 | |
2.3.1.75 | MgCl2 | 5 mM, 17% inhibition | Acinetobacter baylyi ADP1 | |
2.3.1.75 | MnCl2 | 5 mM, 30% inhibition | Acinetobacter baylyi ADP1 |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.75 | 0.029 | - |
palmitoyl-CoA | pH 7.4, 35°C | Acinetobacter baylyi ADP1 |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.3.1.20 | lipid particle | - |
Acinetobacter baylyi ADP1 | - |
- |
2.3.1.22 | lipid particle | mainly | Acinetobacter sp. | - |
- |
2.3.1.22 | membrane | small enzyme amout | Acinetobacter sp. | 16020 | - |
2.3.1.22 | additional information | determination of subcellular distribution, overview | Acinetobacter sp. | - |
- |
2.3.1.75 | cytoplasm | partially | Acinetobacter baylyi ADP1 | 5737 | - |
2.3.1.75 | lipid droplet | - |
Acinetobacter baylyi ADP1 | 5811 | - |
2.3.1.75 | membrane | cytoplasm membrane | Acinetobacter baylyi ADP1 | 16020 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.3.1.20 | 38100 | - |
2 * 38100, gel filtration | Acinetobacter baylyi ADP1 |
2.3.1.20 | 94100 | - |
gel filtration | Acinetobacter baylyi ADP1 |
2.3.1.22 | 38000 | - |
recombinant enzyme, gel filtration, monomeric form | Acinetobacter sp. |
2.3.1.22 | 53000 | - |
1 * 53000, recombinant enzyme, SDS-PAGE | Acinetobacter sp. |
2.3.1.22 | 53000 | - |
2 * 53000, recombinant enzyme, SDS-PAGE | Acinetobacter sp. |
2.3.1.22 | 94100 | - |
recombinant enzyme, gel filtration, dimeric form | Acinetobacter sp. |
2.3.1.75 | 53000 | - |
2 * 53000, SDS-PAGE | Acinetobacter baylyi ADP1 |
2.3.1.75 | 94100 | - |
gel filtration | Acinetobacter baylyi ADP1 |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.20 | Acinetobacter baylyi ADP1 | Q8GGG1 | expression in Escherichia coli | - |
2.3.1.22 | Acinetobacter sp. | - |
gene atfA | - |
2.3.1.22 | Acinetobacter sp. ADP1 | - |
gene atfA | - |
2.3.1.75 | Acinetobacter baylyi ADP1 | Q8GGG1 | bifunctional wax ester synthase/acyl coenzyme A:diacylglycerol acyltransferase, EC 2.3.1.75 and EC 2.3.1.20 resp., enzyme also has acyl-CoA:monoacylglycerol acyltransferase activity, expression in Escherichia coli | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.3.1.20 | recombinant enzyme | Acinetobacter baylyi ADP1 |
2.3.1.22 | recombinant soluble enzyme from Escherichia coli strain BL21(DE3) by cation exchange, hydrophobic interaction, and anion exchange chromatography, 18.7fold | Acinetobacter sp. |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.3.1.22 | 0.118 | - |
purified recombinant enzyme, substrates palmitoyl-CoA and 1,3-dipalmitoylglycerol | Acinetobacter sp. |
2.3.1.22 | 0.211 | - |
purified recombinant enzyme, substrates palmitoyl-CoA and 1,2-dipalmitoylglycerol | Acinetobacter sp. |
2.3.1.75 | 2 | - |
pH 7.4, 35°C | Acinetobacter baylyi ADP1 |
EC Number | Storage Stability | Organism |
---|---|---|
2.3.1.22 | -70°C, purified recombinant enzyme, no significant loss after several months | Acinetobacter sp. |
2.3.1.75 | -70°C, several months without significant loss of activity | Acinetobacter baylyi ADP1 |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.20 | acyl-CoA + 1-decanol | at 48% of the activity with hexanol | Acinetobacter baylyi ADP1 | ? + CoA | - |
? | |
2.3.1.20 | acyl-CoA + 2-cyclohexylethanol | at 45% of the activity with hexanol | Acinetobacter baylyi ADP1 | ? + CoA | - |
? | |
2.3.1.20 | acyl-CoA + 2-decanol | at 39% of the activity with hexanol | Acinetobacter baylyi ADP1 | ? + CoA | - |
? | |
2.3.1.20 | acyl-CoA + 4-decanol | at 15.6% of the activity with hexanol | Acinetobacter baylyi ADP1 | ? + CoA | - |
? | |
2.3.1.20 | acyl-CoA + cyclododecanol | at 80% of the activity with hexanol | Acinetobacter baylyi ADP1 | ? + CoA | - |
? | |
2.3.1.20 | acyl-CoA + cyclohexandiol | at 4.1% of the activity with hexanol | Acinetobacter baylyi ADP1 | ? + CoA | - |
? | |
2.3.1.20 | acyl-CoA + cyclohexanol | at 32% of the activity with hexanol | Acinetobacter baylyi ADP1 | ? + CoA | - |
? | |
2.3.1.20 | acyl-CoA + cyclohexanone oxime | at 5.2% of the activity with hexanol | Acinetobacter baylyi ADP1 | ? + CoA | - |
? | |
2.3.1.20 | acyl-CoA + hexadecanol | - |
Acinetobacter baylyi ADP1 | ? + CoA | - |
? | |
2.3.1.20 | acyl-CoA + phenol | at 4.1% of the activity with hexanol | Acinetobacter baylyi ADP1 | ? + CoA | - |
? | |
2.3.1.20 | acyl-CoA + phenylethanol | at 99% of the activity with hexanol | Acinetobacter baylyi ADP1 | ? + CoA | - |
? | |
2.3.1.20 | additional information | enzyme also has acyl-CoA-monoacylglycerol acyltransferase activity, sn-1 and sn-3 positions are accepted with higher specificity than sn-2 position | Acinetobacter baylyi ADP1 | ? | - |
? | |
2.3.1.22 | additional information | the bifunctional enzyme catalyzes the reaction of EC 2.3.1.22, acyl coenzyme A:diacylglycerol acyltransferase, and of EC 2.3.1.75, wax ester synthase, overview, linear acyl acceptor specificity, C14-C18 acceptor substrates in the wax ester synthase reaction are preferred, overview | Acinetobacter sp. | ? | - |
? | |
2.3.1.22 | additional information | the bifunctional enzyme catalyzes the reaction of EC 2.3.1.22, acyl coenzyme A:diacylglycerol acyltransferase, and of EC 2.3.1.75, wax ester synthase, overview, linear acyl acceptor specificity, C14-C18 acceptor substrates in the wax ester synthase reaction are preferred, overview | Acinetobacter sp. ADP1 | ? | - |
? | |
2.3.1.22 | palmitoyl-CoA + 1,2-dipalmitoylglycerol | i.e. 1,2-dipalmitin | Acinetobacter sp. | CoA + tripalmitoylglycerol | - |
? | |
2.3.1.22 | palmitoyl-CoA + 1,2-dipalmitoylglycerol | i.e. 1,2-dipalmitin | Acinetobacter sp. ADP1 | CoA + tripalmitoylglycerol | - |
? | |
2.3.1.22 | palmitoyl-CoA + 1,3-dipalmitoylglycerol | i.e. 1,2-dipalmitin | Acinetobacter sp. | CoA + tripalmitoylglycerol | - |
? | |
2.3.1.22 | palmitoyl-CoA + 1,3-dipalmitoylglycerol | i.e. 1,2-dipalmitin | Acinetobacter sp. ADP1 | CoA + tripalmitoylglycerol | - |
? | |
2.3.1.22 | palmitoyl-CoA + 2,3-dipalmitoylglycerol | i.e. 1,2-dipalmitin | Acinetobacter sp. | CoA + tripalmitoylglycerol | - |
? | |
2.3.1.22 | palmitoyl-CoA + 2,3-dipalmitoylglycerol | i.e. 1,2-dipalmitin | Acinetobacter sp. ADP1 | CoA + tripalmitoylglycerol | - |
? | |
2.3.1.75 | 1-decanol + palmitoyl-CoA | 48% the rate of hexacedanol | Acinetobacter baylyi ADP1 | decyl palmitate + CoA | - |
? | |
2.3.1.75 | 2-cyclohexylethanol + palmitoyl-CoA | 44.8% the rate of hexacedanol | Acinetobacter baylyi ADP1 | 2-cyclohexylethyl palmitate + CoA | - |
? | |
2.3.1.75 | 2-decanol + palmitoyl-CoA | 38.8% the rate of hexacedanol | Acinetobacter baylyi ADP1 | dec-2-yl palmitate + CoA | - |
? | |
2.3.1.75 | 4-decanol + palmitoyl-CoA | 15.6% the rate of hexacedanol | Acinetobacter baylyi ADP1 | 4-decyl palmitate + CoA | - |
? | |
2.3.1.75 | cyclododecanol + palmitoyl-CoA | 79.7% the rate of hexacedanol | Acinetobacter baylyi ADP1 | cyclododecyl palmitate + CoA | - |
? | |
2.3.1.75 | cyclohexandiol + palmitoyl-CoA | 4.1% the rate of hexacedanol | Acinetobacter baylyi ADP1 | ? + CoA | - |
? | |
2.3.1.75 | cyclohexanol + palmitoyl-CoA | 32% the rate of hexacedanol | Acinetobacter baylyi ADP1 | cyclohexyl palmitate + CoA | - |
? | |
2.3.1.75 | cyclohexanone oxime + palmitoyl-CoA | 5.2% the rate of hexacedanol | Acinetobacter baylyi ADP1 | ? + CoA | - |
? | |
2.3.1.75 | hexadecanol + palmitoyl-CoA | - |
Acinetobacter baylyi ADP1 | hexadecyl palmitate + CoA | - |
? | |
2.3.1.75 | additional information | no substrate: resorcinol | Acinetobacter baylyi ADP1 | ? | - |
? | |
2.3.1.75 | phenol + palmitoyl-CoA | 4.1% the rate of hexacedanol | Acinetobacter baylyi ADP1 | phenyl palmitate + CoA | - |
? | |
2.3.1.75 | phenylethanol + palmitoyl-CoA | 99% the rate of hexacedanol | Acinetobacter baylyi ADP1 | phenylethyl palmitate + CoA | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.3.1.20 | dimer | 2 * 38100, gel filtration | Acinetobacter baylyi ADP1 |
2.3.1.22 | dimer | 2 * 53000, recombinant enzyme, SDS-PAGE | Acinetobacter sp. |
2.3.1.22 | monomer | 1 * 53000, recombinant enzyme, SDS-PAGE | Acinetobacter sp. |
2.3.1.75 | dimer | 2 * 53000, SDS-PAGE | Acinetobacter baylyi ADP1 |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.1.22 | acyl coenzyme A:diacylglycerol acyltransferase | - |
Acinetobacter sp. |
2.3.1.22 | More | cf. EC 2.3.1.75 | Acinetobacter sp. |
2.3.1.22 | wax ester synthase/acyl coenzyme A:diacylglycerol acyltransferase | - |
Acinetobacter sp. |
2.3.1.22 | WS/DGAT | - |
Acinetobacter sp. |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.3.1.22 | 35 | - |
assay at | Acinetobacter sp. |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.3.1.20 | 40 | 45 | - |
Acinetobacter baylyi ADP1 |
2.3.1.75 | 40 | 45 | - |
Acinetobacter baylyi ADP1 |
2.3.1.75 | 65 | - |
no activity above | Acinetobacter baylyi ADP1 |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.3.1.20 | 4 | 40 | - |
Acinetobacter baylyi ADP1 |
2.3.1.75 | 4 | 40 | stable | Acinetobacter baylyi ADP1 |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.3.1.20 | 8 | - |
phosphate buffer | Acinetobacter baylyi ADP1 |
2.3.1.22 | 8 | - |
in phosphate buffer | Acinetobacter sp. |
2.3.1.75 | 8 | - |
phosphate buffer | Acinetobacter baylyi ADP1 |