BRENDA - Enzyme Database

Direct glutaminyl-tRNA biosynthesis and indirect asparaginyl-tRNA biosynthesis in Pseudomonas aeruginosa PAO1

Akochy, P.M.; Bernard, D.; Roy, P.H.; Lapointe, J.; J. Bacteriol. 186, 767-776 (2004)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
6.1.1.18
-
Pseudomonas aeruginosa
6.1.1.23
overproduced from the cloned gene in Pseudomonas aeruginosa
Pseudomonas aeruginosa
6.3.5.6
overexpression in Escherichia coli
Pseudomonas aeruginosa
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
6.1.1.23
ATP + L-aspartate + tRNAAsn
Pseudomonas aeruginosa
the product L-aspartyl-tRNAAsn is transamidated by amidotransferase to form Asn-tRNAAsn. Synthesis of Asn-tRNA via the indirect pathway
AMP + diphosphate + L-aspartyl-tRNAAsn
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
6.1.1.18
Pseudomonas aeruginosa
-
-
-
6.1.1.23
Pseudomonas aeruginosa
-
PAO1
-
6.3.5.6
Pseudomonas aeruginosa
-
PAO1
-
Purification (Commentary)
EC Number
Commentary
Organism
6.1.1.18
partial
Pseudomonas aeruginosa
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.1.1.18
ATP + L-glutamine + tRNAGln
-
659057
Pseudomonas aeruginosa
AMP + diphosphate + L-glutaminyl-tRNAGln
-
-
-
?
6.1.1.23
ATP + L-aspartate + tRNAAsn
the product L-aspartyl-tRNAAsn is transamidated by amidotransferase to form Asn-tRNAAsn. Synthesis of Asn-tRNA via the indirect pathway
659057
Pseudomonas aeruginosa
AMP + diphosphate + L-aspartyl-tRNAAsn
-
-
-
?
6.3.5.6
ATP + Asp-tRNAAsn + glutamine
-
659057
Pseudomonas aeruginosa
ADP + phosphate + Asn-tRNAAsn + glutamate
-
-
-
?
6.3.5.6
ATP + Glu-tRNAGln + glutamine
the enzyme has no Glu-tRNAGln substrate in its host, but it can transamidate heterologous Glu-tRNAGln
659057
Pseudomonas aeruginosa
ADP + Gln-tRNAGln + glutamate + phosphate
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
6.3.5.6
trimer
-
Pseudomonas aeruginosa
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
6.1.1.18
-
Pseudomonas aeruginosa
6.1.1.23
overproduced from the cloned gene in Pseudomonas aeruginosa
Pseudomonas aeruginosa
6.3.5.6
overexpression in Escherichia coli
Pseudomonas aeruginosa
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
6.1.1.23
ATP + L-aspartate + tRNAAsn
Pseudomonas aeruginosa
the product L-aspartyl-tRNAAsn is transamidated by amidotransferase to form Asn-tRNAAsn. Synthesis of Asn-tRNA via the indirect pathway
AMP + diphosphate + L-aspartyl-tRNAAsn
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
6.1.1.18
partial
Pseudomonas aeruginosa
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.1.1.18
ATP + L-glutamine + tRNAGln
-
659057
Pseudomonas aeruginosa
AMP + diphosphate + L-glutaminyl-tRNAGln
-
-
-
?
6.1.1.23
ATP + L-aspartate + tRNAAsn
the product L-aspartyl-tRNAAsn is transamidated by amidotransferase to form Asn-tRNAAsn. Synthesis of Asn-tRNA via the indirect pathway
659057
Pseudomonas aeruginosa
AMP + diphosphate + L-aspartyl-tRNAAsn
-
-
-
?
6.3.5.6
ATP + Asp-tRNAAsn + glutamine
-
659057
Pseudomonas aeruginosa
ADP + phosphate + Asn-tRNAAsn + glutamate
-
-
-
?
6.3.5.6
ATP + Glu-tRNAGln + glutamine
the enzyme has no Glu-tRNAGln substrate in its host, but it can transamidate heterologous Glu-tRNAGln
659057
Pseudomonas aeruginosa
ADP + Gln-tRNAGln + glutamate + phosphate
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
6.3.5.6
trimer
-
Pseudomonas aeruginosa