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Literature summary extracted from

  • Akochy, P.M.; Bernard, D.; Roy, P.H.; Lapointe, J.
    Direct glutaminyl-tRNA biosynthesis and indirect asparaginyl-tRNA biosynthesis in Pseudomonas aeruginosa PAO1 (2004), J. Bacteriol., 186, 767-776.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.18
-
 Pseudomonas aeruginosa
6.1.1.23 overproduced from the cloned gene in Pseudomonas aeruginosa Pseudomonas aeruginosa
6.3.5.6 overexpression in Escherichia coli Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.23 ATP + L-aspartate + tRNAAsn Pseudomonas aeruginosa the product L-aspartyl-tRNAAsn is transamidated by amidotransferase to form Asn-tRNAAsn. Synthesis of Asn-tRNA via the indirect pathway AMP + diphosphate + L-aspartyl-tRNAAsn
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.18 Pseudomonas aeruginosa
-
-
-
6.1.1.23 Pseudomonas aeruginosa
-
 PAO1
-
6.3.5.6 Pseudomonas aeruginosa
-
 PAO1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.18 partial Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.18 ATP + L-glutamine + tRNAGln
-
 Pseudomonas aeruginosa AMP + diphosphate + L-glutaminyl-tRNAGln
-
 ?
6.1.1.23 ATP + L-aspartate + tRNAAsn the product L-aspartyl-tRNAAsn is transamidated by amidotransferase to form Asn-tRNAAsn. Synthesis of Asn-tRNA via the indirect pathway Pseudomonas aeruginosa AMP + diphosphate + L-aspartyl-tRNAAsn
-
 ?
6.3.5.6 ATP + Asp-tRNAAsn + glutamine
-
 Pseudomonas aeruginosa ADP + phosphate + Asn-tRNAAsn + glutamate
-
 ?
6.3.5.6 ATP + Glu-tRNAGln + glutamine the enzyme has no Glu-tRNAGln substrate in its host, but it can transamidate heterologous Glu-tRNAGln Pseudomonas aeruginosa ADP + Gln-tRNAGln + glutamate + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.5.6 trimer
-
 Pseudomonas aeruginosa

Synonyms

EC Number Synonyms Comment Organism
6.1.1.18 GlnRS
-
 Pseudomonas aeruginosa
6.1.1.23 Aspartyl-tRNA synthetase
-
 Pseudomonas aeruginosa
6.1.1.23 AspRS
-
 Pseudomonas aeruginosa
6.1.1.23 nondicriminating AspRS
-
 Pseudomonas aeruginosa
6.3.5.6 AdT
-
 Pseudomonas aeruginosa